Ion binding compositions

ABSTRACT

The present invention provides methods and compositions for the treatment of ion imbalances. In particular, the invention provides core-shell compositions and pharmaceutical compositions thereof. Methods of use of the core-shell compositions for therapeutic and/or prophylactic benefits are disclosed herein. Examples of these methods include the treatment of phosphate imbalance disorders, hypertension, chronic heart failure, end stage renal disease, liver cirrhosis, chronic renal insufficiency, fluid overload, or sodium overload.

INTRODUCTION

Ion selective sorbents have been used in human therapy to correct disorders in electrolyte balance, in conditions such as hyperphosphatemia, hyperoxaluria, hypercalcemia, and hyperkalemia. Hyperphosphatemia occurs in patients with renal failure, whose kidneys no longer excrete enough phosphate ions to compensate exogenous phosphate uptake in the diet. This condition leads to high serum phosphate concentration and high calcium×phosphate product. Although the etiology is not fully demonstrated, high calcium×phosphate product has been held responsible for soft tissue calcification and cardiovascular disease. Cardiovascular disease is the cause of death in almost half of all dialysis patients.

Aluminum, calcium, and, more recently, lanthanum salts have been prescribed to control phosphate ion absorption in the gastrointestinal (GI) tract and restore systemic phosphate levels back to normal. However these salts liberate soluble aluminum and calcium cations in the GI tract, which are then partially absorbed into the blood stream. Aluminum absorption can cause serious side effects such as aluminum bone disease and dementia; high calcium uptake leads to hypercalcemia and puts patients at risk for coronary calcification.

Metal-free phosphate binders such as strong base ion-exchanger materials, Dowex and Cholestyramine resins, have been suggested for use as phosphate binders. However, their low capacity of binding requires high dosage that is not well tolerated by patients.

Amine functional polymers have been described as phosphate or oxalate binders. For example, see U.S. Pat. Nos. 5,985,938; 5,980,881; 6,180,094; 6,423,754; and PCT publication WO 95/05184. Renagel, a crosslinked polyallylamine resin, is a phosphate sequestering material introduced in the market as a metal-free phosphate binder. In vitro phosphate binding of Renagel is approximately 6 mmol/gm in water and 2.5 mmol/gm when measured in 100 mM sodium chloride solution. The recommended dosage for the targeted patient population is typically between 5 gms/day to 15 gms/day to keep the phosphate concentration below 6 mg/dL. Published phase I clinical trials on Renagel, performed on healthy volunteers, indicate that 15 gms of Renagel decrease the phosphate urinary excretion from a baseline of 25 mmole to 17 mmole, the difference being excreted in the feces as free and polymer-bound phosphate. From these data, the in vivo capacity range can be established at 0.5-1 mmol/gm, which is much less than the in vitro capacity of 2.5 mmol/gr measured in saline. Considering only the in vitro binding capacity of Renagel measured in saline, a dosage of 15 gm of the 2.5 mmol/gm phosphate binder would bind the entire phosphorous content of the average American diet, i.e. 37 mmol/day. The discrepancy between the in vitro binding capacity and the documented low in vivo binding capacity has a negative impact on the therapeutic benefit of the drug since more resin is needed to bring the serum phosphate to a safe range.

This loss of capacity of ion-exchange resins is not limited to Renagel when used in the complex environment of the GI tract environment. For example, cation exchange resins in the sodium or ammonium form have been administered to patients with hyperkalemia. The exchange capacity of these resins were measured from isolated feces and found to be about 20% of the in vitro capacity (Agarwal, R., Gastroenterology, 1994, 107, 548-571).

Although generally safe from a toxicological perspective, the large dose and inconvenience associated with taking multigram amounts of resin (e.g., up to 15 gms/day for Renagel and considerably higher in the cases of sodium-binding resins) argues for the need to improve resin capacity. As an example, even in reported safety studies of the Renagel binder, patients have noted gastrointestinal discomfort at doses as low as 1.2-2.0 gm/day for an 8 week treatment period. Patients receiving 5.4 gm of Renagel/day were discontinued from treatment due to adverse events such as GI discomfort in 8.9% of the cases (Slatapolsky, et al Kidney Int. 55:299-307, 1999; Chertow, et al Nephrol Dial Transplant 14:2907-2914, 1999). Thus, an improvement in in vivo binding capacity that translates to lower, better tolerated dosing would be a welcome improvement in resin-based therapies.

As a result of these considerations there is still a great need for safe, high-capacity binders that selectively remove ions from the body with a lower drug dosage and a better patient compliance profile.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides core-shell compositions and pharmaceutical compositions thereof. The core-shell compositions of the present invention comprise of a core component and a shell component. In a preferred embodiment, the core of the core-shell composition is a polymer and can preferentially bind one or more target solutes, e.g., in the gastrointestinal (GI) tract of an animal. In another preferred embodiment, the permeability of the shell component is modified based on the external environment.

Another aspect of the invention provides methods for treatment of patients using the core-shell compositions described herein. In a preferred embodiment, the core-shell compositions are used to remove target solutes from the GI tract. Examples of target solutes that can be removed from the GI tract include, but are not limited to, phosphate, oxalate, sodium, chloride, protons, potassium, iron, calcium, ammonium, magnesium, urea, and creatinine. In another preferred embodiment, the compositions described herein are used in the treatment of hyperphosphatemia, hypocalcemia, hyperparathyroidism, depressed renal synthesis of calcitriol, tetany due to hypocalcemia, renal insufficiency, ecotopic calcification in soft tissues, hypertension, chronic heart failure, end stage renal disease, liver cirrhosis, fluid overload, sodium overload, hyperkalemia, metabolic acidosis, renal insufficiency, and anabolic metabolism.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a schematic representation of one embodiment of a core-shell composition.

FIG. 2 depicts the solute binding profile as a function of time for some embodiments of the invention.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides core-shell polymeric compositions. Also, methods and kits for using these compositions are described herein.

Core Shell Compositions

One aspect of the invention is a core-shell composition comprising a core component and a shell component. In a preferred embodiment, the core-shell composition is a polymeric composition and the core component can preferentially bind one or more target solutes, e.g., in the gastrointestinal (GI) tract of an animal. The term “animal” and “animal subject” as used herein includes humans as well as other mammals.

As shown in FIG. 1, in one embodiment, the core-shell composition comprises core-shell particles with a core component 2 and a shell component 4. The core component is capable of preferentially binding one or more target solutes and the shell component has a higher permeability for the target solutes compared to the permeability for one or more competing solutes. The size of the arrows in FIG. 1 corresponds to the magnitude of the permeability of the solutes. In preferred embodiments, the shell of the core-shell composition is essentially not disintegrated during the period of residence and passage through the gastro-intestinal tract.

The term “target solute” as used herein means a solute that is preferentially bound and/or retained by the core component of the core-shell composition. It is preferred that the target solute has a higher permeability across the shell compared to one or more competing solutes. In preferred embodiments, the shell preferentially allows contact of the target solute with the core. Target solutes include both ions and non-ionic molecules. The ions include both organic and inorganic ions. The ions also include hydrophilic ions, hydrophobic ions, hydrophilic neutral molecules, and hydrophobic neutral molecules. Examples of anionic target solutes include phosphate, chloride, bicarbonate, and oxalate ions. Examples of cationic target solutes include protons, sodium, potassium, magnesium, calcium, ammonium, and other heavy metal ions. Target solutes also include toxins such as uremic toxins. Examples of uremic toxins include urea, creatinine, and classes of compounds such as ribonucleosides, guanidines, polyols, peptides, purines, pyrimidines. See Vanholder et al., Kidney International, vol. 63, (2003), 1934-1943.

In one embodiment, the target solutes excludes high molecular weight molecules like proteins, polysaccharides, and cell debris whose molecular weight are greater than about 50,000 daltons, preferably greater than 5000 daltons. Target solutes also include non-ionic molecules such as organic and inorganic neutral molecules, as well as hydrophilic and hydrophobic neutral molecules. For example, the non-ionic molecules include biological toxins, enzymes, metabolites, drugs, bodily secretions, hormones, etc. Typically, the toxins bound by the compositions disclosed herein are less than about 10,000 daltons, preferably less than 5000 daltons, and even more preferably less than 2000 daltons. The compositions disclosed herein with suitable properties could be used to treat toxicities caused by uremia, drug overdoses or exposure to toxins such as biological toxins or chemical contaminants.

In one embodiment, the core-shell particle preferably binds target solutes, excluding bile acids. In another embodiment, the core-shell particle preferably binds a bile acid and one additional target solute which is not a bile acid.

The term “competing solute” as used herein means solutes that compete with the target solute for binding to a core component, but that are not desired to be contacted and/or bound to the core component. Typically, the competing solute for a core-shell composition depends on the binding characteristics of the core and/or the permeability characteristics of the shell component. A competing solute can be prevented from contacting and/or binding to a core-shell particle due to the preferential binding characteristics of the core component and/or the decreased permeability of the shell component for the competing solute from the external environment. Typically, the competing solute has a lower permeability from the external environment across the shell compared to that of the target solute. For example, for a core-shell composition with a core component that preferably binds phosphate ions, an example of a competing solute is bile acids and fatty acids. The bile acids and fatty acids can be kept away from the core and not be allowed to bind to the core due to the permeability barrier created by the shell component that is more permeable to phosphate ions than bile acids.

In one embodiment, the target solute is hydrophilic ions. The core-shell polymeric compositions which have hydrophilic ions as target solutes are preferably used to remove hydrophilic ions from physiological fluids. More preferably, such core-shell compositions have utility in selectively removing phosphate, oxalate and/or chloride anions. In another embodiment, the hydrophilic ions removed are sodium and/or potassium ions.

It is preferred that the core component of the core-shell particles preferentially binds at least one target solute. The term “preferential binding” and its grammatical equivalents are used herein to describe the favored binding of the target solute to the core component and/or core-shell particles compared to the binding of competing solutes. The preferential binding of target solute can be due to a higher binding affinity for target solutes compared to competing solutes. Preferential binding also encompasses an increased amount of binding of target solutes by the core component, compared to the binding of competing solutes. In some of the preferred embodiments, the core-shell particles bind a greater amount of target solute compared to the core by itself in the absence of the shell. The increased amount of binding can be from about 5% to 100%. It is preferred that the increase in binding of target solute in the presence of the shell compared to the amount bound in the absence of the shell is about 10% or greater, more preferred is about 25% or greater, even more preferred is about 50% or greater, and most preferred is about 95% or greater.

It is also preferred that the core-shell particles retain a significant amount of the bound target solute. The term “significant amount” as used herein is not intended to mean that the entire amount of the bound target solute is retained. It is preferred that at least some of the bound solute is retained, such that a therapeutic and/or prophylactic benefit is obtained. Preferred amounts of bound target solutes that are retained range from about 5% to about 100%. It is preferred that the core-shell compositions retain about 50% of the bound target solute, more preferred is about 75%, and even more preferred is greater than 95%. The period of retention of the bound sodium is preferred to be during the time that the core-shell composition is being used therapeutically and/or prophylactically. In the embodiment in which the core-shell composition is used to bind and remove target solutes from the gastro-intestinal tract, the retention period is preferred to be during the time of residence of the composition in the gastro-intestinal tract. For a topical preparation or a core-shell composition used for a local effect, the retention time is typically the period the composition is present on the topical location or the location that the local effect is desired.

In one embodiment, the core component is composed of polymers containing functional groups with specific binding properties for a given solute, i.e. the target solute. The functional groups with the desired binding properties can be incorporated in the polymer backbone or pendant to the backbone. The binding interactions between the target solutes and the functional groups of the binding core can be of various kinds including, but not limited to, acid-base, coulombic, dipolar, hydrogen bonding, covalent binding, P_(i) interaction, and combinations thereof.

In different embodiments of the invention, the preferential binding between the target solute and the competing solutes can be controlled by the rate of sorption of solutes within the core material or by the rate of permeation of the solutes across the shell component. That is, it is possible to modify the affinity of a target solute for the core component by modifying the overall permeation rate across the particle while keeping the binding core characteristics constant. Also, it is possible to reverse the selectivity for a set of solutes for a given binding core, by creating a permeability coefficient difference in the shell.

Some of the characteristics of the shell membrane and the solutes that influence the permeation of solutes across the core-shell particles are:

-   -   size and shape of the hydrated solute;     -   degree of association/aggregation of the solute (e.g., when         micelles are formed);     -   charge of the solutes;     -   hydration ratio of the shell;     -   mesh size of the shell; and     -   interaction between shell and solutes.

Other parameters also influence the overall mass transfer of solutes to the interior of the core-shell particles:

-   -   specific surface area (i.e. particle diameter);     -   thickness of the shell; and     -   convection current at the outside of the particles.

When there are no chemical interactions between the polymeric composition and the solute, the diffusion can be described by Fick's first law:

$J_{s} = {\frac{P}{l}\left( {C_{o} - C_{i}} \right)}$

-   -   where J_(S) is the solute flux in g/cm²/s;     -   L is the membrane thickness (cm);     -   P is the permeability coefficient in cm²/s; and     -   C₀-C_(i) is the concentration gradient across the membrane.         The permeability coefficient is expressed as:         P=KD     -   where K is a dimension-less parameter (assimilated to the solute         partition coefficient between the membrane and the solution) and     -   D is the solute coefficient in the aqueous solution.         Several models are known to express the permeability coefficient         P, such as the capillary pore model (Renkin equation) and the         free volume model, among others.

In the free volume model, the polymeric composition that makes up the core and/or shell component is considered to be a homogenously hydrated network. The diffusion transport of solutes is considered to occur through fluctuating water-filled spaces within the polymeric network. The free volume diffusion model predicts that D scales with the fraction of polymer in the membrane, φ, and the radius of the hydrated solute, r_(s). A refinement has been proposed (Peppas et al., J. Appl. Polym. Sci., 36, 735-747, 1988) as the hydrodynamic model:

$\frac{D}{D_{0}} = {k_{1}{\exp\left\lbrack {{- k^{\prime}}{r_{s}^{2}\left( \frac{\phi}{1 - \phi} \right)}} \right\rbrack}\mspace{14mu}{diffusion}\mspace{14mu}{model}}$ $\frac{D}{D_{0}} = {{\exp\left\lbrack {{- k_{c}}r_{s}\phi^{3/4}} \right\rbrack}\mspace{14mu}{hydrodynamic}\mspace{14mu}{model}}$

-   -   where D and D₀ are the diffusion coefficients in the membrane         and the aqueous solution, respectively and     -   k₁ is related to the sieving factor, when the geometry of the         solute is the critical parameter that dictates the solute         progression in the core-shell composition and k′ and k_(c) are         undefined structural factors.

For a target solute such as phosphate ions, a typical value of self-diffusion coefficient is 10³¹ ⁵ cm²/s. Based on certain diffusion models, the permeation rate across a micron thick shell membrane is estimated to be extremely fast with respect to the time of use of the resin, typically hours.

If bile acid or fatty acid molecules, as the competing solutes, compete for the same core binding sites as phosphate ions, their self-diffusion coefficient is inversely proportional to their size in solution, which is not so different from one of small ions. Thus, this self-diffusion coefficient may not be enough to create a permeability barrier, if the diffusion is unhindered. Accordingly, in some embodiments, several characteristics of the shell component are tuned so that a permeation difference is established. For example, when the mesh size of the shell material is in the same size range as the solute dimensions, the random walk of the bulkier solute through the shell component is significantly slowed down. For example, experimental studies (Krajewska, B., Reactive and Functional polymers 47, 2001, 37-47) report permeation coefficients in cellulose ester or crosslinked chitosan gel membranes for both ionic and non-ionic solutes shows slowing down of bulkier solutes when mesh size nears solute dimensions. Accordingly, D values can decrease several orders of magnitude depending on the molecular size of the solutes and the polymer volume fraction in the core-shell compositions, the polymer volume fraction in the swollen resin being a good indicator of the mesh size within the composition. Theoretical studies have shown, for example, that mesh size usually scales with φ^(−3/4), φ being the polymer volume fraction in the shell component when swollen in a solution.

In some embodiments, the permeability of the solute is modulated by the degree of interaction between the solute and the shell material. A strong interaction can trap the solute inside the shell component, almost shutting down the migration across the shell. Examples of types of interaction include ionic, covalent, polar, hydrogen bonding, van der Waals, and hydrophobic interactions.

In some embodiments, depending upon the conditions of use and the type of solutes, the ratio between the diffusion coefficient of the target solute and the competing solutes through the shell, is between about 1.1:1 to about 10⁹:1, preferably between about 2:1 to about 10⁶:1.

When the core-shell particles of the invention are used, the solute binding profile as a function of time, of some embodiments, can be schematically represented as depicted in FIG. 2. In a preferred embodiment, the target solute migrates quickly through the shell to be bound to the core material quickly attaining its binding value corresponding to a non-competing mode. In contrast, the competing solute slowly progresses through the shell as a result of its lower permeation rate; it eventually reaches its binding equilibrium value later in time and then displaces the target solute, causing a drop in the target solute binding curve. Preferably the ratio of diffusion coefficients is adjusted so that, at the end of the time of use of the binder (which may correspond to mean residence time of the resin in the GI) is less than about 10% to about 100% of the competing solutes have reached their binding equilibrium value. Preferably less than about 10%, more preferably less than about 50%, and even more preferably less than about 75% of the competing solutes have reached their binding equilibrium value. For the target solutes more than about 10% to about 100% has reached its binding equilibrium value in a non-competing mode. Preferably more than about 25%, more preferably more than about 50%, even more preferably more than about 75% of the target solute has reached its binding equilibrium.

Methods for determining diffusion coefficients are known. For example, see, W. Jost, Diffusion in Solids, Liquids and Gases, Acad. Press, New-York, 1960). For example, the diffusion coefficient of a shell polymer can be measured by casting it as a membrane over a solid porous material, which is then contacted with a physiological solution containing the solutes of interest, and measuring steady state permeation rates of said solutes. Membrane characteristics can then be optimized to achieve the best cooperation in terms of selectivity and permeation rate kinetics. Structural characteristics of the membrane can be varied by modifying, for example, the polymer volume fraction (in the swollen membrane), the chemical nature of the polymer(s), the polymer blend composition (if more than one polymer is used), the formulation with additives such as wetting agents, plasticizers, and the manufacturing process.

Alternatively, if the shell membrane is applied to the core material in a separate coating process, then the selectivity effect provided by the shell can be obtained by measuring the binding capacity for the target solute using the core particles with and without the shell. The increase in selectivity, SI, can be simply expressed as the ratio of those two values, i.e. SI=CB_(core-shell)/CB_(core), where CB represent the capacity of binding (i.e mole of solute per unit weight of particle). Preferably, SI is between about 1.05 to about 10⁴, even more preferably from about 1.1 to about 10².

In some embodiments, the shell is a film-forming polymer. In another embodiment, the shell polymer forms a crosslinked gel with a three-dimensional network structure where chains are crosslinked through covalent bonds, ionic or other bonds. In yet another embodiment, the shell material is chemically identical to the binding core material, but the crosslink density increases outward from core to shell. In another embodiment, the shell material adopts a “brush” configuration, wherein individual polymer strands are covalently attached to the core material at their termini. In this embodiment, the mesh size can be dictated by the density of chains anchored onto the surface and by the chain molecular weight. The polymer brush design variables that control the permeability of polymer brushes to solutes of various sizes and/or weights are known in the art. For example, see WO 0102452 (and references therein).

Permeability is also controlled by the interaction of the solute with the shell. A strong and, preferably, irreversible interaction of the shell with the competing solutes can trap the competing solutes within the encapsulating shell, slowing down their progression inward. One means of quantifying the degree of interaction between a solute and the shell is the free energy of mixing, particularly the free enthalpy of mixing, which can be predicted by solubility parameters. Solubility parameters provide a numerical method of predicting the extent of interaction between materials, particularly liquids and polymers. This model predicts that compounds with dissimilar solubility parameters will not co-dissolve and consequently can go through the membrane unhindered in the absence of size sieving effect. Conversely, compounds with similar solubility parameters may form a molecular solution and can be retained. Further, while solubility parameters poorly describe ionic interactions, charged solutes generally are retained by shell material of opposite charge. Also, the combination of hydrophobic and ionic interactions can be used to provide strong, often irreversible, interactions with competing solutes, resulting in higher sorption selectivity for the target solutes which display neither a hydrophobic or an ionic character.

The shell material can be chosen from natural or synthetic polymers, optionally crosslinked, alone or in combination with small molecules functional additives such as wetting agents, plasticizers, permeability enhancers, solvents, moisturizing agents, pigment, and/or dyes.

Naturally-occuring or semi-synthetic polymers include: cellulose ethers (ethyl cellulose, methyl cellulose and their copolymers), cellulose esters (cellulose acetate, cellulose propionate, cellulose phthalate, and their copolymers), hydroxypropyl cellulose, hydroxyl ethyl cellulose, chitosan, deacetylated chitosan, and the like. Other examples of possible shell materials are listed in the table below:

TABLE 1 Acrylics Gums, vegetable Polyvinyl acetate Aquacoat ® aqueous dispersions Halocarbon Polyvinyl pyrrolidone Aquateric ® enteric coatings Hydrocarbon resins Polyvinyl alcohol Cellulose Acetate Hydroxy Propyl Cellulose Polyvinyl chloride Cellulose Acetate Butyrate Hydroxy Propyl Methyl Polyvinylacetate phthalate Cellulose Cellullose Acetate Phthalate Hydroxy Propyl Methyl Polyvinylidene chloride Cellulose Phthalate Caseinates Kynar ® fluoroplastics Proteins Chlorinated rubber Maltodextrins Rubber, synthetic Coateric ® coatings Methyl Cellulose Shellac Coating butters Microcrystalline wax Silicone Daran ® latex Milk solids Starches Dextrins Molasses Stearines Ethyl Cellulose Nylon Sucrose Enterics Opadry ® coating systems Surfactants Eudragits ® polymethacrylates Paraffin wax Surelease ® coating systems Ethylene Vinyl Acetate Phenolics Teflon ® fluorocarbons Fats Polylactides Waxes Fatty Acids Polyamino acids Zein Gelatin Polyethylene Glycerides Polyethylene glycol Gums, vegetable

Examples of suitable synthetic polymers that can be used in the shell component include polymers produced by free radical polymerization of ethylenic monomers (acrylic and methacrylic, styrenic, dienic, vinylic), polycondensates (polyester, polyamides, polycarbonate, polysulfone), polyisocyanate, polyurea, epoxy resins, and the like.

Shell deposition over the core material can be carried out using coating techniques such as spraying, pan coating, fluidized bed (Wurster coating units), dipping, solvent coacervation, polyelectrolyte inter-complex layers, and the “layer by layer” encapsulation process. Other encapsulation processes are also applicable. For example, see Encapsulation and Controlled Release by R.A. Stephenson (Editor), David R. Karsa (Editor), 1993.

The shell can comprise several layers of distinct composition, of which one can be an enteric coating (e.g. Eudragit acrylic polymers) that disintegrates and/or solubilizes at a specific location of the GI tract. Examples of suitable enteric coatings are known in the art, for example see Remington: The Science and Practice of Pharmacy by A. R. Gennaro (Editor), 20^(th) Edition, 2000.

The shell can also be grown on the core component through chemical means, for example by:

-   -   chemical grafting of shell polymer to the core using living         polymerization from active sites anchored onto the core polymer;     -   interfacial reaction, i.e., a chemical reaction located at the         core particle surface, such as interfacial polycondensation; and     -   using block copolymers as suspending agents during the core         particle synthesis.

The interfacial reaction and use of block polymers are preferred techniques when chemical methods are used. In the interfacial reaction pathway, typically, the periphery of the core particle is chemically modified by reacting small molecules or macromolecules on the core interface. For example, an amine containing ion-binding core particle is reacted with a polymer containing amine reactive groups such as epoxy, isocyanate, activated esters, halide groups to form a crosslinked shell around the core.

In another embodiment, the shell is first prepared using interfacial polycondensation or solvent coacervation to produce capsules. The interior of the capsule is then filled up with core-forming precursors to build the core within the shell capsule.

In some embodiments, using the block copolymer approach, an amphiphilic block copolymer can be used as a suspending agent to form the core particle in an inverse or direct suspension particle forming process. When an inverse water-in-oil suspension process is used, then the block copolymer comprises a first block soluble in the continuous oil phase and another hydrophilic block contains functional groups that can react with the core polymer. When added to the aqueous phase, along with core-forming precursor, and the oil phase, the block copolymer locates to the water-in-oil interface and acts as a suspending agent. The hydrophilic block reacts with the core material, or co-reacts with the core-forming precursors. After the particles are isolated from the oil phase, the block copolymers form a thin shell covalently attached to the core surface. The chemical nature and length of the blocks can be varied to vary the permeation characteristics of the shell towards solutes of interest.

In some embodiments, the shell thickness can be between about 0.002 micron to about 50 micron, preferably about 0.005 micron to about 20 microns. Preferably the shell thickness is more than about 1 micron, more preferred is more than about 10 micron, even more preferred is more than about 20 micron, and most preferred is more than about 40 micron. Preferably the shell thickness is less than about 50 micron, more preferred is less than about 40 micron, even more preferred is less than about 20 micron, and most preferred is less than about 10 micron.

In another embodiment, the shell to core weight ratio comprises between about 0.01% to about 50%, preferably between about 0.2% to about 10%. The size of the core-shell particles typically range from about 200 nm to about 2 mm, preferably being about 500 μm. Preferably the size of the core-shell particles are more than about 1 μm, more preferred is more than about 100 μm, even more preferred is more than about 200 μm, and most preferred is more than about 400 μm. Preferably the size of the core-shell particles are less than about 500 μm, more preferred is less than about 400 μm, even more preferred is less than about 200 μm, and most preferred is less than about 100 μm.

The binding selectivity of the core can be assessed by standard methods. One method consists of measuring the binding capacity of the target solute in a simple model solution with non interfering species, Cm, and in a simulant medium (Cs), and calculating a selectivity index as SI=Cs/Cm. The core-shell particles of the invention are expected to have selectivity indexes SI significantly higher than those reported for known prior-art sorbent resins.

In one embodiment, the permeability of the shell changes as a function of time. In particular, the permeability of the shell may change over time when used in vivo. For example, in certain applications it is preferable to either diminish or conversely increase the permeability to target solutes over time during residence in a GI tract. For example, the resin could bind a hydrophilic ionic solute at a certain location of the GI tract at a rate controlled by the solute concentration in equilibrium with the resin at that location. As the resin travels down the GI tract, the local target solute concentration may vary as a result of dilution or solute transport across the gut membrane. In this embodiment, the shell material is engineered to respond to such concentration or other physiological changes in the GI, so that its permeability is altered; more specifically, the permeability of the shell maybe decreased during its journey through the GI so that hydrophilic ions are no longer able to cross the shell membrane, during the later period of the core-shell composition's residence in the GI tract. This embodiment also applies to more hydrophobic solutes such as bile acids. In the case of bile acid sequesterants, studies have shown that the poor binding rate in vivo is caused by the release of bile acids past the ileum segment of the gut. At that point, bile acids are almost quantitatively reabsorbed by the mucosa, so that the binding equilibrium is shifted and the sequestering capacity is lowered. In this embodiment, the shell component has a permeability trigger that decreases the permeability of the shell to bile acids, when the core-shell resin passes the ileum so that the overall capacity is conserved.

One manner of achieving this loss of permeability to hydrophilic ions involves decreasing or even eliminating the free volume of permeation of the shell membrane. The free volume of permeation of the membrane can be modified by controlling the hydration rate of the shell. In this manner, it is possible to almost shut down the rate of permeation by inducing a shell collapse. While there are many ways to induce such a phase change, the preferred approach consists of rendering the membrane material increasingly hydrophobic so that the hydration rate decreases almost to zero. This can be accomplished through several ways depending upon the type of triggering mechanism. For example the triggering mechanism can be by pH change. The pH profile of the gastrointestinal tract presents several domains which may change as a function of time, but show some invariants indicated below (Fallinborg et al. Aliment. Pharm. Therap. (1989), 3, 605-613):

TABLE 2 GI tract segment Ph range Stomach 1-2 Duodenum - distal small intestine 6-7 Ceacum-ascending colon   7-5.5 Transverse-descending colon 5.5-6   Feces 6.5

Shell polymers exhibiting a chain collapse in any of these pH regions would be prone to permeability changes. For instance, core-shell particles suitable for binding a solute selectively in the stomach and keeping it in the particle core while the particles are moving down the small and large intestine, would display high permeability to solutes at low pH and very low permeability at neutral pH. This can be done by having a shell polymer with hydrophobic groups and groups that ionize subject to pH change. For example, polymers built from hydrophobic monomers (e.g. long chain alcohol (meth)arylates, Nalkyl (meth)acrylamide), and basic monomers that ionize at low pH and remain neutral beyond their pKa (e.g. vinyl-pyridine, dialkylaminoethyl (meth)acrylamide) can be used. The relationship between pH and shell swelling ratio, and hence permeability, can be controlled by the balance of hydrophobic monomers and ionizable monomers. Examples of such systems are reported in the literature. For example, see Batich et al, Macromolecules, 26, 4675-4680.

A further drop in permeability may be desirable when pH increases (e.g. from ileum to colon) to prevent bound electrolytes being released as the resin environment changes. This can be achieved where the shell material switches from a hydrated state to a collapsed, impermeable state as the pH gets slightly basic. In such embodiments, shell polymers typically contain a balanced amount of hydrophobic and acidic monomers. Such systems are extensively described in the literature. For example, see Kraft et al. Langmuir, 2003, 19, 910-915; Ito et al, Macromolecule, (1992), 25, 7313-7316.

Another means of changing shell permeability is by passive absorption. As described above, components present in the GI tract, whether coming from the diet, produced as diet digest metabolites, from secretion, etc. are susceptible to adsorption on and within the shell in a quasi-irreversible manner and this adsorption may modify the permeability pattern by inducing membrane collapse. The vast majority of these GI tract components is negatively charged and shows various levels of hydrophobicity. Some of these species have an amphiphilic character, such as fatty acids, bile acids, phospholipids, and biliary salts and behave as surfactants. Surfactants can adsorb non-specifically to surfaces through hydrophobic interactions, ionic interaction and combinations thereof. In the context of the present invention, this phenomenon can be used to change the permeability of the resin upon the course of binding to these surfactants during the resin's residence in the GI tract.

For example, fatty acids and bile acids both form insoluble complexes when mixed with positively charged polymers. For example, see Kaneko et al, Macromolecular Rapid Communications, 2003, 24(13), 789-792). Both types of molecules present similarities with synthetic anionic surfactants, and numerous studies report the formation of insoluble complexes between anionic surfactants and cationically charged polymers. For example, see Chen, L. et al, Macromolecules (1998), 31(3), 787-794. In this embodiment, the shell material is selected from copolymers containing both hydrophobic and cationic groups, so that the shell forms a complex, preferably a tight complex, with anionically charged hydrophobs typically found in the GI tract, such as bile acids, fatty acids, bilirubin and related compounds. Suitable compositions also include polymeric materials described as bile acids sequestering agents, such as those reported in U.S. Pat. No. 5,607,669, U.S. Pat. No. 6,294,163, U.S. Pat. No. 5,374,422, Figuly et al, Macromolecules, 1997, 30, 6174-6184. The formation of this complex induces a shell membrane collapse which in turn lowers or shuts down the permeation rate across the said membrane.

The shell permeability may also be modulated by enzymatic transformation. In one embodiment the shell comprises a hydrophobic backbone with pendant hydrophilic entities that are cleaved off via an enzymatic reaction in the gut. As the enzymatic reaction proceeds, the polymer membrane becomes more and more hydrophobic, and turns from a high swollen, high permeability material to a fully collapsed low hydration membrane with minimal permeability. Hydrophilic entities can be chosen amongst natural substrates of enzymes commonly secreted in the GI tract. Such entities include amino acids, peptides, carbohydrates, esters, phosphate esters, oxyphosphate monoesters, O- and S-phosphorothioates, phosphoramidates, thiophosphate, azo groups and other similar entities. Examples of enteric enzymes which can be used to chemically alter the shell polymer include, but are not limited to, lipases, phospholipases, carboxylesterase, glycosidases, azoreductases, phosphatases, amidases and proteases.

In some embodiments, the core material is chosen from polymer compositions with the desired ion binding properties. Examples of suitable polymers material include, but are not limited to:

-   -   1) anion binding materials such as amine functional polymers         such as those described in U.S. Pat. Nos. 5,985,938; 5,980,881;         6,180,094; 6,423,754; and PCT publication WO 95/05184 and     -   2) cation exchange polymers, such as those with acid functional         groups such as carboxylate, phosphonate, sulfate, sulfonate,         sulfamate functional polymers and combinations thereof.

Core-shell compositions that include anion binding materials are useful for the binding and removal from GI tract of phosphate, chloride, bicarbonate, and oxalate ions. The cation exchange polymers have utility in binding and removal of physiologically important cations such as protons, sodium, potassium, magnesium, calcium, ammonium, and the like or heavy metals which cause poisoning.

Examples of other suitable polymers for the core component are described in the following co-pending patent applications: 1) Polyamine Polymers, filed on Nov. 3, 2003, application Ser. No. 10/701,385 and 2) Crosslinked Amine Polymers, filed on Mar. 22, 2004, application Ser. No. 10.806,495.

Further examples of compositions that can be used in the core component include the phosphate binders in PCT publications WO 94/19379, WO 96/25440, WO 01/28527, WO 02/85378, WO 96/39156, WO 98/42355, WO 99/22743, WO 95/05184, WO 96/21454, and WO 98/17707; U.S. Pat. Nos. 5,698,190; 5,851,518; 5,496,545; 5,667,775; 6,083,495; and 6,509,013; and European Patent Application 01200604.5.

Aluminum, calcium, and lanthanum salts are used as phosphate binders. Examples of inorganic metal salts used as phosphate binders include aluminum carbonate, aluminum hydroxide gel (Amphojel®), calcium carbonate, calcium acetate (PhosLo), and lanthanum carbonate (Fosrenol). In one embodiment, the core-shell particle comprises of a core component comprising of a metal phosphate binder, such as aluminum carbonate, aluminum hydroxide gel, calcium carbonate, calcium acetate, and lanthanum carbonate.

In one embodiment, the core component has sodium ion binding properties. Suitable polymers that can be used in the core so as to impart the core sodium-binding properties include crown ethers. Crown ethers exhibit selectivity for certain alkali metals over others, based mainly on the hole-size of the crown ether size and the size of the metal. Crown ethers of the type 15-18 are preferred for use in sodium ion binding core components. Also, other suitable compositions for sodium binding properties are described in co-pending patent application entitled “Methods and Compositions for Treatment of Ion Imbalances,” filed on Mar. 30, 2004, application Ser. No. 10/814,527.

Uses of the Core-Shell Compositions

In one aspect, the invention provides methods of preferentially binding solutes in a mammal, comprising the step of administering to the mammal a therapeutically effective amount of core-shell compositions. Core shell compositions that bind hydorphillic cations and/or anions can be used to control ion homeostasis and treat electrolyte balance disorders in phosphate (hyperphosphatemia), oxalate (calcium oxalate kidney stones, oxaluria), sodium (hypertension), potassium (hyperkalemia), chloride (acidosis), or to remove toxic metals or oxidative anions in cases of poisoning.

The core-shell compositions with anion exchange resins are particularly useful in the binding and excretion of negatively charged ions from the body. Core-shell compositions can also be used to bind metallic ions. These compositions can be administered orally to bind and remove from an animal various negatively charged entities and metallic species from the gastro-intestinal tract. In one embodiment, the core-shell compositions of the present invention are used to remove phosphate, oxalate, bile acids, small molecules, proteins, metallic ions such as those comprised within the 6th and the 11th groups and 4th and 6th periods of the Periodic Table, also including the Lanthanoids and the Actanoids.

In some embodiments, the core-shell compositions with polyvicinalamines, such as those described in co-pending U.S. patent application Ser. No. 10/701,385; entitled Polyamine Polymers: 29329-703, filed on Nov. 3, 2003 are useful in the treatment of renal diseases, hyperphosphatemia, and the removal of bile acids, oxalates and iron from the gastrointestinal tract.

In some embodiments, the core-shell compositions are used in the treatment of phosphate imbalance disorders. The term “phosphate imbalance disorder” as used herein refers to conditions in which the level of phosphorus present in the body is abnormal. One example of a phosphate imbalance disorder includes hyperphosphatemia. The term “hyperphosphatemia” as used herein refers to a condition in which the element phosphorus is present in the body at an elevated level. Typically, a patient is often diagnosed with hyperphosphatemia if the blood phosphate level is, for example, above 4.5 milligrams per deciliter of blood and/or glomerular filtration rate is reduced to, for example, more than about 20%

Other diseases that can be treated with the methods and compositions of the present invention include hypocalcemia, hyperparathyroidism, depressed renal synthesis of calcitriol, tetany due to hypocalcemia, renal insufficiency, and ecotopic calcification in soft tissues including calcifications in joints, lungs, kidney, conjuctiva, and myocardial tissues. Also, the present invention can be used to treat ESRD and dialysis patients. In one embodiment, the core-shell compositions are used for prophylactic treatment of diseases.

The core-shell compositions described herein can also be used to treat diseases wherein a reduction in physiological levels of salt is desired. The core-shell compositions, depending on the ion binding properties of the core component, can be used to remove cations such as sodium and/or anions such as chloride.

In one embodiment, the core-shell compositions of the present invention are used to treat metallic poisoning, like iron poisoning. Iron poisoning typically is a result of children inadvertently taking iron supplement tablets. In iron overdose, binding of iron to oral charcoal, bicarbonate, deferoxamine, or magnesium hydroxide are typical treatments. Gastric lavage and profuse oral fluids are used to try to flush out the iron tablets. Non-absorbable core-shell compositions with iron chelating properties can be used for removal of metallic iron.

Depending on the properties of the core and/or shell components, the core-shell compositions of the present invention also show utility in binding dietary oxalate in patients who suffer from hyperoxaluria, i.e. abnormally high levels of oxalate in the urine. Elevated urine oxalate levels are one of the causes of calcium-stone formation (i.e., kidney stones). Most calcium stones are composed of calcium oxalate, either alone or in combination with calcium phosphate or calcium urate. Elevated urinary oxalate levels can result from excessive dietary intake of oxalate (dietary oxaluria), gastrointestinal disorders that lead to malabsorption of oxalate (enteric oxaluria), or an inherited enzyme deficiency that results in excessive metabolism of oxalate (primary hyperoxaluria or PH). Dietary and enteric oxaluria can be treated with diet restriction or modifications to restrict intake of foods with high oxalate content. However patient compliance is often difficult owing to the wide distribution of oxalate and purine derivatives in many foods. Calcium carbonate tablets (500-650 mg/tablet; 3 tablets per meal) can also be taken to bind and remove intestinal oxalate, but again patient compliance is difficult owing to the amount of calcium carbonate needed. Core components made of polyvicinalamines, such as those described co-pending U.S. patent application Ser. No. 10/701,385; entitled Polyamine Polymers: 29329-703, filed on Nov. 3, 2003, have high binding constants for oxalate and can be used to remove oxalate from the gastro-intestinal tract and subsequently lower the risk of kidney stone formation.

In the present invention, the core-shell compositions can be co-administered with other active pharmaceutical agents depending on the condition being treated. This co-administration can include simultaneous administration of the two agents in the same dosage form, simultaneous administration in separate dosage forms, and separate administration. For example, for the treatment of hyperphosphatemia, the core-shell compositions can be co-administered with calcium salts which are used to treat hypocalcemia resulting from hyperphosphatemia. The calcium salt and core-shell composition can be formulated together in the same dosage form and administered simultaneously. Alternatively, the calcium salt and core-shell composition can be simultaneously administered, wherein both the agents are present in separate formulations. In another alternative, the calcium salt can be administered just followed by the core-shell composition, or vice versa. In the separate administration protocol, the core-shell composition and calcium salt may be administered a few minutes apart, or a few hours apart, or a few days apart.

The term “treating” as used herein includes achieving a therapeutic benefit and/or a prophylactic benefit. By therapeutic benefit is meant eradication, amelioration, or prevention of the underlying disorder being treated. For example, in a hyperphosphatemia patient, therapeutic benefit includes eradication or amelioration of the underlying hyperphosphatemia. Also, a therapeutic benefit is achieved with the eradication, amelioration, or prevention of one or more of the physiological symptoms associated with the underlying disorder such that an improvement is observed in the patient, notwithstanding that the patient may still be afflicted with the underlying disorder. For example, administration of core-shell compositions to a patient suffering from renal insufficiency and/or hyperphosphatemia provides therapeutic benefit not only when the patient's serum phosphate level is decreased, but also when an improvement is observed in the patient with respect to other disorders that accompany renal failure and/or hyperphosphatemia like ectopic calcification and renal osteodistrophy. For prophylactic benefit, the core-shell compositions may be administered to a patient at risk of developing hyperphosphatemia or to a patient reporting one or more of the physiological symptoms of hyperphosphatemia, even though a diagnosis of hyperphosphatemia may not have been made.

The pharmaceutical compositions of the present invention include compositions wherein the core-shell compositions are present in an effective amount, i.e., in an amount effective to achieve therapeutic or prophylactic benefit. The actual amount effective for a particular application will depend on the patient (e.g., age, weight, etc.), the condition being treated, and the route of administration. Determination of an effective amount is well within the capabilities of those skilled in the art, especially in light of the disclosure herein.

The effective amount for use in humans can be determined from animal models. For example, a dose for humans can be formulated to achieve circulating and/or gastrointestinal concentrations that have been found to be effective in animals.

The dosages of the core-shell compositions in animals will depend on the disease being, treated, the route of administration, the physical characteristics of the patient being treated, and the composition of the core and shell components. Dosage levels of the core-shell compositions for therapeutic and/or prophylactic uses can be from about about 0.5 gm/day to about 30 gm/day. It is preferred that these polymers are administered along with meals. The compositions may be administered one time a day, two times a day, or three times a day. Most preferred dose is about 15 gm/day or less. A preferred dose range is about 5 gm/day to about 20 gm/day, more preferred is about 5 gm/day to about 15 gm/day, even more preferred is about 10 gm/day to about 20 gm/day, and most preferred is about 10 gm/day to about 15 gm/day.

In some embodiments, the amount of target solute bound and/or retained by the core-shell particles is greater than the amount if the core component is used in the absence of the shell. Hence, the dosage of the core component in some embodiments is lower when used in combination with a shell compared to when the core is used without the shell. Hence, in some embodiments of the core-shell pharmaceutical compositions, the amount of core component present in the core-shell pharmaceutical composition is less than the amount that is administered to an animal in the absence of the shell component.

Preferably, the core-shell compositions used for therapeutic and/or prophylactic benefits can be administered alone or in the form of a pharmaceutical composition. The pharmaceutical compositions comprise of the core-shell compositions, one or more pharmaceutically acceptable carriers, diluents or excipients, and optionally additional therapeutic agents. The compositions can be administered by injection, topically, orally, transdermally, or rectally. Preferably, the core-shell composition or the pharmaceutical composition comprising the core-shell composition is administered orally. The oral form in which the core-shell composition is administered can include powder, tablet, capsule, solution, or emulsion. The therapeutically effective amount can be administered in a single dose or in a series of doses separated by appropriate time intervals, such as hours.

Pharmaceutical compositions for use in accordance with the present invention may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen. Suitable techniques for preparing pharmaceutical compositions of the core-shell compositions are well known in the art.

In addition to the uses of the core-shell compositions described herein in the gastro-intestinal tract, these compositions can also be used for producing local effects in other parts of the body, for example in topical formulations for local effects on the skin or in systemic formulations for producing local effects in particular organs, like the liver or the heart.

EXAMPLES Example 1

Synthesis of Core-Shell Crosslinked Polyallylamine Particles

In this process, spherical particles were formed by an inverse suspension procedure wherein a prepolymer (polyallylamine) is crosslinked with epichlorohydrine. A block copolymer was used to impart mechanical stability to the droplets during the crosslinking reaction and provide a shell membrane chemically anchored to the core particle.

General Procedure for Block Copolymers Synthesis

The block copolymers were prepared by RAFT living free radical polymerization method, using a dithiocarbazide compound as a reversible chain transfer agent (CTA) and a diazonitrile free radical initiator (AMVN) indicated below:

Synthesis of Poly(n-butyl acrylate-co-t-butyl acrylate) First Block

n-Butyl acrylate (25 g, 195 mmol) and t-butyl acrylate (25 g, 195 mmol) were combined with the CTA (173:1 Monomer:CTA, 616 mg, 2.26 mmol)) and AIBN (18.6 mg, 0.113 mmol). The monomer to CTA ratio is fixed so that the theoretical number average molecular weight (Mn) is 20,000 g·mol at 90% conversion. The solution was stirred while purging with Ar for 20 minutes at room temperature. After this time, it was heated to 65° C. under Ar while stirring for 3 hours and then cooled to room temperature. ¹H NMR in CDCl₃ showed 87% conversion based on disappearance of monomer. The crude polymer was dissolved in 50 ml of acetone and precipitated into 900 ml of a 9:1 (v/v) methanol: water solution. After several hours, the polymeric oil had separated to the bottom and the top layer was discarded. The polymeric oil was dried in vacuum to yield 44 g (88% yield) of extremely thick yellow oil. ¹H NMR (300 MHz, CDCl₃):δ=4.15-3.95 (2H, bm), 2.45-2.05 (2H, bm). 1.95-1.75 (1H, bm), 1.60-1.5 (5H, bm), 1.5-1.3 (11H, bm), 0.93 (3H, t). GPC (THF, polystyrene standards): Mn=25900; PDI=1.13. GPC (DMF, polyethyleneglycol standards): Mn=6600; PDI=1.58.

Following this procedure, 4 different first blocks were prepared, which are listed in TABLE 3 as Example 1-1 to 1-4.

TABLE 3 Molecular weight Example Identification Composition (g/mol) 1-1 nBA1tBA1_20k n-butyl acrylate-co-t-butyl 20,000 acrylate 50/50 mol-% 1-2 nDiBA1tBA1_20k N,N-di-n-butyl acrylamide- 20,000 co-t-butyl acrylate 50/50 mol-% 1-3 nBA1tBA1_50k nbutyl acrylate-co-t-butyl 50,000 acrylate 50/50 mol-% 1-4 nDiBA1tBA1_50k N,N-di-n-butyl acrylamide- 50,000 co-t-butyl acrylate 50/50 mol-% Synthesis of Poly[(n-butyl acrylate-co-t-butyl acrylate)-b-(N,N-dimethylacrylamide-co-glycidyl methacrylate)]

Theoretical Mn=20,000 1^(st) block and Mn=5000 2^(nd) block at 80% con. A solution of poly(n-butyl acrylate-co-t-butyl acrylate) terminated with the CTA (2.53 ml, 40 wt % in DMF) and a solution of AMVN (48.1 μl, 0.00736 mmol, 4 wt % in DMF) were combined manually. The mixture was then purged with Ar for 20 minutes. While stirring at room temperature, N,N-dimethylacrylamide (27.5 μl, 0.267 mmol) and a solution of glycidyl methacrylate (14.3 μl, 0.0296 mmol, 30 wt % in DMF) were added. The solution temperature was then raised to 55° C. over 30 minutes while stirring. At this time, N,N-dimethylacrylamide (10.3 μl, 0.100 mmol) and a solution of glycidyl methacrylate (5.4 μl, 0.0111 mmol, 30 wt % in DMF) were added via a robot. Every 10 minutes for the next 4 hours N,N-dimethylacrylamide (10.3 μl, 0.100 mmol) and a solution of glycidyl methacrylate (5.4 μl, 0.0111 mmol, 30 wt % in DMF) were added while the solution stirred under Ar at 55° C. After all additions had been completed, the solution was stirred for an additional 2 hours under Ar at 55° C. and then cooled to room temperature. The crude polymer was dissolved in 2 ml acetone and precipitated into 30 ml water. The resulting mixture was centrifuged at 1000 rpm for 60 minutes and the upper water layer then removed. The polymeric powder was washed with an additional 10 ml of water, centrifuged, and the water layer removed. The resulting wet powder was dried under vacuum at 30° C. to give a viscous liquid. Subsequent lyophilization provided 1.19 g (92% yield) of a sticky solid. GPC (DMF, polyethyleneglycol standards): Mn=8500; PDI=2.10.

Similar procedures were used to make block copolymers of various length and chemical compositions which are reported in the following tables, TABLES 4 and 5.

TABLE 4 Library: Plate 1 (ID: 100436) Unit: mg Row Col nDiBA1tBA1 20k nDiBA1tBA1 50k DMF THF AMVN GMA DMA A 1 1065.7 0.0 754.2 707.3 2.0 45.8 287.3 A 2 915.9 0.0 709.1 665.0 1.7 78.7 493.8 A 3 714.9 0.0 648.6 608.2 1.3 122.8 770.8 A 4 1065.7 0.0 754.2 707.3 2.0 45.8 287.3 A 5 915.9 0.0 709.1 665.0 1.7 78.7 493.8 A 6 714.9 0.0 648.6 608.2 1.3 122.8 770.8 B 1 982.0 0.0 810.5 760.1 1.8 116.8 190.1 B 2 798.9 0.0 806.5 756.3 1.5 190.1 309.2 B 3 581.8 0.0 801.7 751.8 1.1 276.8 450.4 B 4 982.0 0.0 810.5 760.1 1.8 116.8 190.1 B 5 798.9 0.0 806.5 756.3 1.5 190.1 309.2 B 6 581.8 0.0 801.7 751.8 1.1 276.8 450.4 C 1 0.0 897.8 622.9 584.1 0.7 38.6 242.0 C 2 0.0 770.4 585.8 549.3 0.6 66.2 415.3 C 3 0.0 600.1 536.1 502.7 0.4 103.1 647.0 C 4 0.0 897.8 622.9 584.1 0.7 38.6 242.0 C 5 0.0 770.4 585.8 549.3 0.6 66.2 415.3 C 6 0.0 600.1 536.1 502.7 0.4 103.1 647.0 D 1 0.0 826.5 670.8 629.0 0.6 98.3 160.0 D 2 0.0 671.1 668.2 626.7 0.5 159.7 259.8 D 3 0.0 487.7 665.3 623.9 0.4 232.1 377.6 D 4 0.0 826.5 670.8 629.0 0.6 98.3 160.0 D 5 0.0 671.1 668.2 626.7 0.5 159.7 259.8 D 6 0.0 487.7 665.3 623.9 0.4 232.1 377.6 General Design and Variations from Example (mol:mol ratios) Starting Block (45.5 wt % solns): Rows A,B = 20k 1:1 N,N-di-n-Butyl Acrylamide:t-Butyl Acylate Rows C,D = 50k 1:1 N,N-di-n-Butyl Acrylamide:t-Butyl Acylate Initiator: AMVN Temperature: 60 C. 2nd Block Compostion: Rows A, C = 1:9 GMA:DMA Rows B, D = 3:7 GMA:DMA Block Target Mn: A1, B1, A4, B4 = 5k A2, B2, A5, B5 = 10k A3, B3, A6, B6 = 20k C1, D1, C4, D4 = 12.5k C2, D2, C5, D5 = 25k C3, D3, C6, D6 = 50k 2nd Block Method: Columns 1, 2, 3 = Batch Addition Columns 4, 5, 6 = Spot Addition

TABLE 5 Library: Plate 1 (ID: 100369) Unit: mg Row Col GMA DMF nBA1tBA1 20k DMA AIBN A 1 39.8 1566.0 963.2 249.2 1.2 A 2 72.5 1458.0 842.7 454.2 1.0 A 3 116.8 1311.6 679.3 732.2 0.8 A 4 39.8 1566.0 963.2 249.2 1.2 A 5 72.5 1458.0 842.7 454.2 1.0 A 6 116.8 1311.6 679.3 732.2 0.8 B 1 74.2 1604.6 935.9 206.6 1.2 B 2 132.1 1532.2 800.2 368.0 1.0 B 3 206.6 1439.1 625.7 575.5 0.8 B 4 74.2 1604.6 935.9 206.6 1.2 B 5 132.1 1532.2 800.2 368.0 1.0 B 6 206.6 1439.1 625.7 575.5 0.8 General Design (mol:mol ratios) Starting Block: 20k 1:1 n-Butyl Acrylate:t-Butyl Acylate Initiator: AIBN Temperature: 65 C. 2nd Block Compostion: Row A = 1:9 GMA:DMA Row B = 2:8 GMA:DMA 2nd Block Target Mn: Columns 1, 4 = 5k Columns 2, 5 = 10k Columns 3, 6 = 20k 2nd Block Method: Columns 1, 2, 3 = Batch Addition Columns 4, 5, 6 = Spot Addition General Procedure for the Synthesis of Core/Shell Crosslinked Polyallylamine Particles:

Preparation of polyallylamine (PAA) solution: Polyallylamine hydrochloride (Mw 15,000) was dissolved in water, and NaOH was added to neutralize 25 mol % of hydrochloride. The concentration of polyallyamine hydrochloride in solution was 33 wt. %.

Preparation of diblock copolymer solution: diblock copolymer was dissolved in toluene at 5 wt. %.

Preparation of core/shell particles: To 15 ml glass reactor was charged PAA solution, diblock copolymer solution, and toluene, and some typical solution compositions as shown in Tables 4-9. The mixture was emulsified with an Ultra-Turrax for 30 seconds and a magnetic stir bar was put into the suspension. The suspension was stirred and heated at 60° C. for 30 minutes and epichlorohydrin (10 mol % based on amine groups) was added. The suspension was further stirred at 60° C. for 8 hours and then cooled to room temperature.

Purification of core/shell particles: To the reaction mixture above, methanol (10 mL) was added and white particles precipitated out. The mixture was shaken for 30 minutes and centrifuged. The white particles separated from supernatant solution and collected. The white particles were further washed with methanol (10 mL×2) and water (10 mL×3) by repeating the same shake/centrifuge procedure. Finally the particles were freeze-dried for three days.

Example 2

Synthesis of 1,3-Diaminopropane/epichlorohydrin Crosslinked Beads (Referred to herein as: Bead-Pi-4-s)

The reaction vessel used was a 3-liter, three necked round bottom flask with four side baffles, equipped with an oil heating bath, cold-water reflux condenser, and mechanical stirrer with a 3 inch propeller. To this reaction vessel is introduced a solution of 1,3-diaminopropane (90.2 g, 1.21 mole) dissolved in 90.2 g of water, surfactant (branched dodecylbenzene sulfonic acid sodium salt, 6.4 g dissolved in 100 g of water), and 1 Kg of toluene. This initial charge is agitated to 600 rpm for 2 minutes and then lowered to 300 rpm for 10 minutes before the first addition of epichlorohydrin. This speed is maintained through out the experiment. The solution was heated to 80° C. and maintained at this temperature throughout the experiment.

Into a separate vessel, a 40 mass % solution of epichlorohydrin in toluene was prepared. Using a syringe pump, 1.2 equivalents of epichlorohydrin (134.7 g, (1.45 mole)) was added over a 3 hour period. The reaction was continued for an additional 2 hours before adding 0.75 equivalents of sodium hydroxide (36.5 g (0.91 mole)) in a 40 weight % solution. The sodium hydroxide solution was added to the reaction via a syringe pump over a 2.5-hour period. The reaction was maintained at 80° C. for a further 8 hours. The beads were purified by removing the toluene, washing with 1000 ml of acetone, followed by methanol, and then a 20% solution of NaOH (to remove the surfactant), and twice more with deionized water. The beads were freeze dried for 3 days to give a fine white powder weighing at 160 g (92% yield), and having a mean diameter of 93 μm.

Synthesis of 1.3-Diaminopropane/epichlorohydrin Crosslinked Beads (Referred to herein as: Bead-Pi-3-s)

The procedure described above was used with 1 equivalent of epichlorohydrin.

Synthesis of Water Swollen Crosslinked Beads Prepared with 1,3-Diaminopropane/epichlorohydrin in the Presence of Surfactant (Referred to herein as: Bead-Pi-5-s)

The procedure described above for the preparation of beads from 1,3-diaminopropane/epichlorohydrin was reproduced exactly up to stage 2. After the reaction flask had cooled to room temperature, the stirring was stopped. The beads settle to the bottom of the flask. The clear toluene layer was decanted from the reaction and replaced by fresh toluene to remove unreacted epichlorohydrin. This procedure was repeated 4 times and washing with a total of 3000 ml of toluene. Through out this process the beads were not allowed to dry out. The total weight of the solution was made to 756 g by adding toluene to give a 21 wt-% solution of bead suspended in toluene.

Example 3

Preparation of ethyl cellulose shell/1,3 di-amino propane epichlorohydrine Crosslinked Core Particle

The beads obtained from Example 2 are spray-coated with an ethyl cellulose polymer shell using a Wurster fluid bed coater 2″-4″/6″ Portable Unit. The fluidized bed unit is operated so that an average 5 microns thick coating is deposited on the core particles, using a 30 wt-% solid aqueous emulsion (Aquacoat® ECD, FMC corp.).

Example 4

Binding Capacity in a Digestion Mimic

This procedure was used to mimic the conditions of use of a phosphate binder drug and measure the binding characteristics of the polymer for phosphate (target solute) in the presence of other metabolites (competing solutes). A liquid meal was prepared and the meal was artificially digested in the presence of pepsin and pancreatic juice. The sequence of addition of enzymes and the pH profile were controlled so that the digestion process was simulated down to the jejunum level. An aliquot of the digested meal mimic is centrifuged and the supernatant assayed for phosphate.

An aliquot of dried resin of weight P(gr), was mixed under gentle agitation with a fixed volume, V(ml), of a meal digest solution with a phosphate ion concentration of C_(start)(mM). After resin equilibration, the solution was decanted by centrifugation and the supernatant analyzed for residual phosphate concentration by ionic chromatography, C_(eq)(mM). The binding capacity was calculated as BC (mmol/gr)=V. (C_(start)−C_(eq))/P.

A. Core/Shell Crosslinked Polyallylamine Particles

Procedures described in Example 1 were implemented in a library format of 4×6 reactors, where the nature of the block copolymer was varied from well to well, as indicated in Tables 6-9. Entries correspond to the weight of chemicals used in each reaction well and to the phosphate binding capacity measured in the meal digest fluid. A Selectivity Index (SI) was computed to measure the phosphate binding relative to the core material (i.e. crosslinked polyallylamine, Renagel). When SI was greater than 1, the core-shell material bound more phosphate than the corresponding core polymer on a weight basis. The SI values for the polymers are included in Tables 6-9.

Results are shown in Tables 6-9. Results collated in this series of examples show that the core-shell particles of the invention display higher binding for phosphate over bare, non-encapsulated particles in simulated fluid representative of the real conditions of use. Some of the best performing core-shell materials are then assessed in binding phosphate in the ex-vivo aspirates from human intestinal content.

TABLE 6 Library 100411 Library: Plate 1 Unit: ul Row Col toluene di-block s PAA s ECH di-block Pstart (mM) Peq (mM) BC (mmol/gr) SI(−) 1 1 281 1860 750 22.6 369_A1 7.6 2.98 1.85 1.09 1 2 281 1860 750 22.6 369_A2 7.6 2.41 2.08 1.22 1 3 281 1860 750 22.6 369_A4 7.6 2.99 1.84 1.08 1 4 281 1860 750 22.6 369_A5 7.6 2.85 1.90 1.12 2 1 281 1860 750 22.6 369_B1 7.6 3.15 1.78 1.05 2 2 281 1860 750 22.6 369_B2 7.6 2.35 2.10 1.23 2 3 281 1860 750 22.6 369_B4 7.6 2.76 1.94 1.14 2 4 281 1860 750 22.6 369_B5 7.6 2.86 1.90 1.12 Polyallylamine 7.6 3.35 1.70 1.00 core Diblock is dispensed as a 5 wt-% solution in toluene

TABLE 7 Library: Plate 1 (ID: 100482) Unit: mg Block copolymers Row Col 436_B4 436_B5 436_B6 436_D4 436_D5 436_D6 Pstart m(M) Peq (mM) BC(mmol/gr) SI(−) 1 1 81.00 0.00 0.00 0.00 0.00 0.00 1 2 0.00 81.00 0.00 0.00 0.00 0.00 13.41 6.32 2.84 1.02 1 3 0.00 0.00 81.00 0.00 0.00 0.00 13.41 6.05 2.94 1.06 1 4 0.00 0.00 0.00 81.00 0.00 0.00 13.41 5.53 3.16 1.14 1 5 0.00 0.00 0.00 0.00 81.00 0.00 13.41 6.33 2.83 1.02 1 6 0.00 0.00 0.00 0.00 0.00 81.00 13.41 4.57 3.54 1.28 2 1 40.50 0.00 0.00 0.00 0.00 0.00 13.41 6.66 2.70 0.97 2 2 0.00 40.50 0.00 0.00 0.00 0.00 13.41 5.55 3.15 1.13 2 3 0.00 0.00 40.50 0.00 0.00 0.00 13.41 5.36 3.22 1.16 2 4 0.00 0.00 0.00 40.50 0.00 0.00 13.41 4.98 3.37 1.22 2 5 0.00 0.00 0.00 0.00 40.50 0.00 13.41 4.82 3.44 1.24 2 6 0.00 0.00 0.00 0.00 0.00 40.50 13.41 3.96 3.78 1.36 3 1 81.00 0.00 0.00 0.00 0.00 0.00 13.41 5.70 3.08 1.11 3 2 0.00 81.00 0.00 0.00 0.00 0.00 13.41 7.09 2.53 0.91 3 3 0.00 0.00 81.00 0.00 0.00 0.00 13.41 5.72 3.08 1.11 3 4 0.00 0.00 0.00 81.00 0.00 0.00 13.41 6.57 2.74 0.99 3 5 0.00 0.00 0.00 0.00 81.00 0.00 13.41 6.40 2.80 1.01 3 6 0.00 0.00 0.00 0.00 0.00 81.00 13.41 6.54 2.75 0.99 4 1 40.50 0.00 0.00 0.00 0.00 0.00 13.41 5.36 3.22 1.16 4 2 0.00 40.50 0.00 0.00 0.00 0.00 13.41 6.07 2.94 1.06 4 3 0.00 0.00 40.50 0.00 0.00 0.00 13.41 5.51 3.16 1.14 4 4 0.00 0.00 0.00 40.50 0.00 0.00 13.41 4.21 3.68 1.33 4 5 0.00 0.00 0.00 0.00 40.50 0.00 13.41 4.96 3.38 1.22 4 6 0.00 0.00 0.00 0.00 0.00 40.50 13.41 4.58 3.53 1.27 Polyallylamine 13.41 6.48 2.77 1.00 core Constant reactor composition (mg) toluene PAA H2O NaOH EPH 1768.95 270.00 511.16 28.84 40.05 Row 1 & 2: 30 sec. sonication time Row 3 & 4: 90 sec. sonication time

TABLE 8 Library Sample Block Copolymer BC (mmol/gr) IC(-) 100516 100516 A1 100436 A1 3.02 1.25 100516 100516 A2 100436 A2 3.44 1.43 100516 100516 A3 100436 A3 3.33 1.38 100516 100516 A4 100436 A4 3.01 1.25 100516 100516 A5 100436 A5 3.29 1.37 100516 100516 A6 100436 A6 3.52 1.46 100516 100516 B1 100436 B1 3.30 1.37 100516 100516 B2 100436 B2 3.60 1.49 100516 100516 B3 100436 B3 3.38 1.40 100516 100516 B4 100436 B4 3.52 1.46 100516 100516 B5 100436 B5 3.74 1.55 100516 100516 B6 100436 B6 3.32 1.38 100516 100516 C1 100436 C1 3.89 1.61 100516 100516 C2 100436 C2 3.54 1.47 100516 100516 C3 100436 C3 2.75 1.14 100516 100516 C4 100436 C4 3.57 1.48 100516 100516 C5 100436 C5 3.53 1.47 100516 100516 C6 100436 C6 2.64 1.09 100516 100516 D1 100436 D1 3.78 1.57 100516 100516 D2 100436 D2 3.57 1.48 100516 100516 D3 100436 D3 3.12 1.29 100516 100516 D4 100436 D4 3.40 1.41 100516 100516 D5 100436 D5 3.75 1.55 Polyallylamine core 2.41 1.00 Constant reactor composition (mg) toluene PAA H₂O NaOH EPH Block cop. 1768.95 270.00 511.16 28.84 40.05 40.05 30 sec. sonication time Library 100517 is identical to 100516 with the exception that the beads were further treated with HCl 1M for 6 hrs @ 60° C. to deprotect the further treated with HCI 1M for terbutylacrylate groups into acrylic acid groups.

TABLE 9 Library Sample Block Copolymer BC(mmol/gr) IC(-) 100517 100517 A1 100436 A1 3.04 1.26 100517 100517 A2 100436 A2 3.30 1.37 100517 100517 A3 100436 A3 3.26 1.35 100517 100517 A4 100436 A4 3.35 1.39 100517 100517 A5 100436 A5 2.86 1.18 100517 100517 B1 100436 B1 3.22 1.33 100517 100517 B2 100436 B2 3.60 1.49 100517 100517 B3 100436 B3 3.64 1.51 100517 100517 B4 100436 B4 3.58 1.48 100517 100517 B5 100436 B5 3.82 1.58 100517 100517 B6 100436 B6 3.62 1.50 100517 100517 C1 100436 C1 3.52 1.46 100517 100517 C2 100436 C2 3.37 1.39 100517 100517 C3 100436 C3 2.86 1.18 100517 100517 C4 100436 C4 3.24 1.34 100517 100517 C5 100436 C5 3.34 1.38 100517 100517 C6 100436 C6 2.22 0.92 100517 100517 D1 100436 D1 3.24 1.34 100517 100517 D2 100436 D2 3.17 1.31 100517 100517 D3 100436 D3 3.21 1.33 100517 100517 D4 100436 D4 3.32 1.38 100517 100517 D5 100436 D5 3.02 1.25 Polyallyamine core 2.42 1.00 Constant reactor composition (mg) toluene PAA H₂O NaOH EPH Block cop. 1768.95 270.00 511.16 28.84 40.05 40.05 30 sec. sonication time Library 100517 is identical to 100516 with the exception that the beads were further treated with HCl 1M for 6 hrs @ 60° C. to deprotect the terbutylacrylate groups into acrylic acid groups. B. Core/Shell Crosslinked 1,3-diaminopropane/epichlorohydrine Particles

Procedures described in Example 2 were implemented in a library format of 4×6 reactors, where the nature of the polymer was varied from well to well, as indicated in Tables 11-18. Entries in the Tables correspond to the weight of chemicals used in each reaction well, and to the phosphate binding capacity measured in the meal digest fluid. A Selectivity Index (SI) was computed as described above. The SI values for the polymers are included in Tables 11-18.

Each example comprised a library of 22 core-shell materials and one core material taken as a reference. The core materials are beads prepared from crosslinked 1,3-diaminopropane/epichlorohydrine as shown in Example 2 (bead 4-s, bead 3-s, and bead 5-s). They were used either as a dry powder (bead 4-s, bead 3-s) or as a slurry in toluene (bead 5-s). The core-shell particles were prepared in semi-continuous reactors arranged in a 4×6 library format. Each reactor had a 3 ml volume, was magnetically stirred, and temperature-controlled. In a typical procedure, the beads were first dispensed, followed by the addition of the selected solvent under magnetic agitation. The reaction temperature was set to 60° C. The shell materials were then robotically dispensed for 4 hours and the 24 reactions kept another 12 hours at the set temperature. The library was then cooled down to ambient temperature and the content of the reactors transferred to 15 ml vials. The core-shell beads were then washed repeatedly with a fresh volume of the same solvent used during the shell coupling reaction, then with isopropanol, and finally with de-ionized water. The particles were finally lyophilized.

The chemical structures of the shell materials used are shown in Table 10.

TABLE 10 Label Name CAS # Structure MW (g/mol) Shell-AH-1 POLY(METHYLVINYL ETHER-ALT- MALEIC ANHYDRIDE)

MW ~ 20,000 Shell-AH-2 POLY(METHYLVINYL ETHER-ALT- MALEIC ANHYDRIDE)

MW ~ 50,000 Shell-AH-3 POLY(METHYLVINYL ETHER-ALT- MALEIC ANHYDRIDE) 9011-16-9

Mn. ~ 80,000 Mw. ~ 216,000 Shell-AH-4 POLY(STYRENE-MALEIC MW = 1600 ANHYDRIDE) 50:50 (molar) Shell-AH-5 POLY(STYRENE-MALEIC MW = 1900 ANHYDRIDE) 75:25 (molar) Shell-AH-6 POLY(STYRENE-co-MALEIC 26762-29-8 Mn = 1600 ANHYDRIDE), CUMENE TERMINATED Shell-AH-7 POLY(STYRENE-co-MALEIC 26762-29-8 Mn = 1700 ANHYDRIDE), CUMENE TERMINATED Shell-AH-8 POLY(STYRENE-co-MALEIC 160611-46-1 Mn = 2300 ANHYDRIDE), PARTIAL FW = 658.8 ISOCTYL ESTER, CUMENE TERMINATED Shell-AH-9 POLY(STYRENE-co-MALEIC 160611-50-7 Av. Mn = 2500 ANHYDRIDE), PARTIAL 2- BUTOXYETHYLESTER, CUMENE TERMINATED Shell-AH-10 POLY(ETHYLENE-co-ETHYL 41171-14-6 / ACRYLATE-co-MALEIC ANHYDRIDE) Shell-AH-11 POLY(STYRENE-co-MALEIC 160611-48-3 Mn ~ 1900 ANHYDRIDE), PARTIAL PROPYL ESTER, CUMENE TERMINATED Shell-AH-12 POLYETHYLENE-graft-MALEIC 106343-08-2 FW ~ 154.2 ANHYDRIDE Shell-AH-13 POLYISOPRENE-graft-MALEIC 139948-75-7 FW = 234.3 ANHYDRIDE Mn ~ 25000 Shell-AH-14 POLY(ETHYLENE-co-BUTYL 64652-60-4 FW = 268.3 ACRYLATE-co-MALEIC ANHYDRIDE) Shell-Cl-1 2-CHLOROETHANESULFONIC ACID SODIUM SALT 15484-44-3

166.56 Shell-Cl-2 3-CHLORO-2- HYDROXYPROPANE- SULFONIC ACID SODIUM SALT 126-83-0

196.59 Shell-Mc-1 DIETHYLENE GLYCOL DIACRYLATE 4074-88-8

214.22 Shell-Mc-2 POLY(ETHYLENE GLYCOL) DIACRYLATE 26570-49-8

Mn. ~ 700 Shell-Mc-3 POLY(ETHYLENE-co- 51541-08-3 FW = 256.3 METHACRYLATE-co- GLYCIDYL METHACRYLATE) Shell-EP-1 2-(3,4-EPOXYCYCLOHEXYL)- ETHYLTRIETHOXYSILANE

288.5 Shell-EP-2 POLY(ETHYL GLYCOL) DIGLYCIDYL ETHER 26403-72-5

526.6 Shell-EP-3 POLY(ETHYL GLYCOL) (200) 26403-72-5 200 DIGLYCIDYL ETHER Shell-EP-4 POLY(ETHYL GLYCOL) (400) 26403-72-5 400 DIGLYCIDYL ETHER Shell-EP-5 POLY(ETHYL GLYCOL) (600) 26403-72-5 600 DIGLYCIDYL ETHER Shell-EP-6 POLY(ETHYL GLYCOL) (1000) 26403-72-5 1000 DIGLYCIDYL ETHER Shell-EP-7 1,3-BUTADIENE DIEPOXIDE 1464-53-5

86.09 Shell-EP-8 3-(1H,1H,7H-DODECA- FLUOROHEPTYLOXY)-1,2 EPOXYPROPANE 799-34-8

388.15 Shell-EP-9 GLYCIDYL 4-NONYLPHENYL ETHER 6178-32-1 276.42

Shell-EP-10 POLY(PROPYLENE GLYCOL) DIGLYCIDYL ETHER 26142-30-3

640 Shell-EP-11 GLYCIDYL HEXADECYL ETHER 15965-99-8 298.51

Shell-EP- 12bis 2-[(4- NITROPHENOXY)METHYL] OXIRANE 5255-75-4

195.18 Shell-EP-12 POLY(BISPHENOL A-co- 25036-25-3 FW = 487.0 EPICHLOROHYDRIN), Mn ~ 355 GLYCICYL END-CAPPED Shell-EP-13 POLY(BISPHENOL A-co- FW = 487.0 EPICHLOROHYDRIN), Mn ~ 1075 GLYCICYL END-CAPPED Shell-EP-14 POLY(BISPHENOL A-co- Mn ~ 1750 EPICHLOROHYDRIN), GLYCICYL END-CAPPED Shell-EP-15 POLY(BISPHENOL A-co- / EPICHLOROHYDRIN), GLYCICYL END-CAPPED Shell-EP-16 POLY(o-CRESYL GLYCIDYL 29690-82-2 FW = 194.2 ETHER)-co-FORMALDEHYDE) Mn ~ 540 Shell-EP-17 POLY(o-CRESYL GLYCIDYL 29690-82-3 FW = 194.2 ETHER)-co-FORMALDEHYDE) Mn ~ 1270 Shell-EP-18 POLY(ETHYLENE-co- 26061-90-5 FW = 170.2 GLYCIDYL METHACRYLATE) Shell-EP-19 BISPHENOL DIGLYCIDYL 1675-54-3 ETHER Shell-EP-20 POLY(DIMETHYLSILOXANE) 130167-23-6 FW = 282.5 DIGLYCIDYL TERMINATED EW ~ 490 Shell-EP-21 POLY[(PHENYL GLYCIDYL / FW = 180.2 ETHER) co-FORMALDEHYDE] Mn ~ 345 Shell-EP-22 POLY[(PHENYL GLYCIDYL 28064-14-4 FW = 180.2 ETHER) co-FORMALDEHYDE] Mn ~ 570 Shell-EP-23 POLY[(PHENYL GLYCIDYL 119345-05-0 FW = 286.4 ETHER) Mn ~ 490 co-DICYCLOPENTADIENE] Shell-EP-24 POLY(EPICHLOROHYDRIN-co 26587-37-1 ETHYLENE OXIDE-co-ALLY GLYCIDYL ETHER Shell-EP-25 CASTOR OIL GLYCIDYL 74398-71-3 ETHER ShelI-EP-26 TETRAPHENYLOLETHANE / GLYCIDYL ETHER Shell-EP-27 EPON RESINS - 828 /

Results are shown in Tables 11-18. Results collated in this series of example show that the core-shell particles of the invention display higher rate of binding for phosphate over bare, non-encapsulated particles in simulated fluid representative of the real conditions of use.

TABLE 11 library Shell- Shell- Shell- bead- ID row column NaOH shell-EP-10 EP-12 EP-16 Mc-3 pi-3 bead-pi-4 bead-pi-5 toluene water BC(mmol/gr) SI(−) 100433 A 1 0.00 1.25 23.75 0.41 0.76 100433 A 2 0.00 1.25 23.75 0.46 0.85 100433 A 3 0.00 1.25 23.75 0.43 0.81 100433 A 4 0.00 1.25 23.75 0.40 0.74 100433 A 5 0.00 1.25 23.75 0.40 0.75 100433 A 6 0.00 1.25 23.75 0.48 0.90 100433 B 1 0.00 1.25 23.75 0.50 0.93 100433 B 2 0.00 1.25 23.75 0.51 0.94 100433 B 3 0.00 1.25 23.75 0.58 1.07 100433 B 4 0.00 1.25 23.75 0.64 1.19 100433 B 5 0.00 1.25 23.75 0.38 0.70 100433 B 6 0.00 1.25 23.75 0.35 0.66 100433 C 1 0.10 1.25 23.66 0.29 0.55 100433 C 2 0.10 1.25 23.66 0.60 1.12 100433 C 3 0.10 1.25 23.66 0.49 0.91 100433 C 4 0.10 1.25 23.66 0.70 1.30 100433 C 5 0.10 1.25 23.66 0.44 0.82 100433 C 6 25.00 0.54 1.00 100433 D 1 1.25 23.75 0.56 1.03 100433 D 2 1.25 23.75 0.63 1.18 100433 D 3 1.25 23.75 0.50 0.94 100433 D 4 1.25 23.75 0.57 1.06 100433 D 5 1.25 23.75 0.43 0.80 100461 A 1 24.00 120.00 2256.00 1.47 1.08 100461 A 2 56.40 141.00 2622.60 1.46 1.07 100461 A 3 95.88 159.80 2940.32 1.45 1.07 100461 A 4 124.00 155.00 2821.00 1.48 1.09 100461 A 5 108.60 108.60 1954.80 1.40 1.03 100461 B 1 32.92 164.60 3094.48 1.26 0.92 100461 B 2 59.04 147.60 2745.36 1.51 1.11 100461 B 3 96.24 160.40 2951.36 1.48 1.09 100461 B 4 129.12 161.40 2937.48 1.46 1.07 100461 B 5 136.10 136.10 2449.80 1.57 1.15 100461 C 1 33.18 165.90 3118.92 1.38 1.02 100461 C 2 54.20 135.50 2520.30 1.39 1.02 100461 C 3 90.36 150.60 2771.04 1.53 1.13 100461 C 4 93.36 116.70 2123.94 1.35 1.00 100461 C 5 148.70 148.70 2676.60 1.52 1.12 100461 C 6 165.10 1.36 1.00 100461 D 1 32.56 162.80 3060.64 1.37 1.01 100461 D 2 75.16 187.90 3494.94 1.24 0.91 100461 D 3 65.94 109.90 2022.16 1.44 1.06 100461 D 4 100.08 125.10 2276.82 1.49 1.10 100461 D 5 166.30 166.30 2993.40 1.47 1.08 100462 A 1 24.21 121.02 2275.23 1.51 1.13 100462 A 2 44.65 111.62 2076.04 1.54 1.15 100462 A 3 64.15 106.91 1967.16 1.45 1.08 100462 A 4 76.94 96.18 1750.48 1.46 1.09 100462 A 5 109.49 109.49 1970.89 1.53 1.14 100462 B 1 22.44 112.20 2109.42 1.49 1.11 100462 B 2 45.46 113.65 2113.93 1.41 1.05 100462 B 3 67.79 112.98 2078.83 1.55 1.16 100462 B 4 89.96 112.46 2046.68 1.55 1.16 100462 B 5 102.12 102.12 1838.21 1.51 1.12 100462 C 1 22.22 111.09 2088.49 1.69 1.26 100462 C 2 42.97 107.44 1998.31 1.76 1.31 100462 C 3 59.03 98.39 1810.28 1.74 1.30 100462 C 4 86.34 107.92 1964.13 1.59 1.18 100462 C 5 108.15 108.15 1946.70 1.48 1.10 100462 C 6 100.30 377.30 1.34 1.00 100462 D 1 20.03 100.15 1882.80 1.60 1.20 100462 D 2 38.24 95.59 1778.01 1.80 1.34 100462 D 3 56.73 94.54 1739.57 1.75 1.31 100462 D 4 85.36 106.70 1941.96 2.00 1.49 100462 D 5 103.19 103.19 1857.49 2.06 1.54

TABLE 12 Shell- Shell- library ID row column AH-11 AH-9 Shell-EP-8 Shell-IC-1 bead-pi-5 ethyl acetate methanol toluene water BC(mmol/gr) SI(−) 100468 A 1 31.70 158.50 2979.80 1.48 0.96 100468 A 2 76.28 190.70 3547.02 1.63 1.06 100468 A 3 106.20 177.00 3256.80 1.56 1.01 100468 A 4 133.20 166.50 3030.30 1.50 0.98 100468 A 5 123.40 123.40 2221.20 1.53 0.99 100468 B 1 28.06 140.30 2637.64 1.70 1.10 100468 B 2 52.48 131.20 2440.32 1.60 1.04 100468 B 3 84.48 140.80 2590.72 1.70 1.10 100468 B 4 106.00 132.50 2411.50 1.73 1.12 100468 B 5 146.40 146.40 2635.20 1.70 1.11 100468 C 1 28.96 144.80 2722.24 1.53 0.99 100468 C 2 50.20 125.50 2334.30 1.45 0.94 100468 C 3 93.48 155.80 2866.72 1.48 0.96 100468 C 4 140.24 175.30 3190.46 1.37 0.89 100468 C 5 188.80 188.80 3398.40 1.19 0.78 100468 C 6 221.10 1.54 1.00 100468 D 1 41.38 206.90 3889.72 1.44 0.94 100468 D 2 61.84 154.60 2875.56 1.49 0.97 100468 D 3 109.62 182.70 3361.68 1.46 0.95 100468 D 4 117.04 146.30 2662.66 1.45 0.94 100468 D 5 148.20 148.20 2667.60 1.46 0.95

TABLE 13 library Shell- Shell- ID row column EP-1 EP-10 Shell-EP-12bis Shell-EP-13 Shell-Mc-2 bead-pi-5 ethyl acetate toluene BC(mmol/gr) SI(−) 100473 A 1 94.02 156.70 2883.28 1.68 1.10 100473 A 2 89.82 149.70 2754.48 1.52 0.99 100473 A 3 114.12 190.20 3499.68 1.57 1.02 100473 A 4 95.76 159.60 2936.64 1.47 0.96 100473 A 5 81.12 135.20 2487.68 1.47 0.96 100473 B 1 98.52 164.20 3021.28 1.49 0.97 100473 B 2 98.76 164.60 3028.64 1.44 0.94 100473 B 3 109.80 183.00 3367.20 1.37 0.89 100473 B 4 92.70 154.50 2842.80 1.41 0.92 100473 B 5 114.24 190.40 3503.36 1.46 0.96 100473 C 1 90.18 150.30 2765.52 1.46 0.95 100473 C 2 90.00 150.00 2760.00 1.43 0.94 100473 C 3 82.74 137.90 2537.36 1.41 0.92 100473 C 4 99.12 165.20 3039.68 1.35 0.88 100473 C 5 106.32 177.20 3260.48 1.36 0.89 100473 C 6 212.40 1.53 1.00 100473 D 1 90.84 151.40 2785.76 1.24 0.81 100473 D 2 100.68 167.80 3087.52 1.42 0.93 100473 D 3 113.82 189.70 3490.48 1.45 0.95 100473 D 4 105.36 175.60 3231.04 1.46 0.95 100473 D 5 90.30 150.50 2769.20 1.51 0.99 100474 A 1 90.18 150.30 2765.52 1.64 1.19 100474 A 2 86.88 144.80 2664.32 1.42 1.03 100474 A 3 101.94 169.90 3126.16 1.26 0.91 100474 A 4 100.92 168.20 3094.88 1.36 0.99 100474 A 5 94.32 157.20 2892.48 1.47 1.07 100474 B 1 88.02 146.70 2699.28 1.36 0.98 100474 B 2 95.70 159.50 2934.80 1.37 0.99 100474 B 3 89.88 149.80 2756.32 1.48 1.07 100474 B 4 109.02 181.70 3343.28 1.40 1.02 100474 B 5 86.46 144.10 2651.44 1.43 1.04 100474 C 1 84.60 141.00 2594.40 1.42 1.03 100474 C 2 89.52 149.20 2745.28 1.45 1.05 100474 C 3 84.72 141.20 2598.08 1.48 1.07 100474 C 4 112.02 186.70 3435.28 1.44 1.04 100474 C 5 104.58 174.30 3207.12 1.42 1.03 100474 C 6 216.20 1.38 1.00 100474 D 1 94.50 157.50 2898.00 1.48 1.07 100474 D 2 110.40 184.00 3385.60 1.42 1.03 100474 D 3 102.18 170.30 3133.52 1.72 1.25 100474 D 4 87.84 146.40 2693.76 1.54 1.12 100474 D 5 97.86 163.10 3001.04 1.53 1.11 100480 A 1 29.76 148.80 2797.44 1.32 0.95 100480 A 2 77.96 194.90 3625.14 1.18 0.85 100480 A 3 102.24 170.40 3135.36 0.95 0.68 100480 A 4 133.28 166.60 3032.12 0.79 0.57 100480 A 5 143.90 143.90 2590.20 0.80 0.57 100480 B 1 32.08 160.40 3015.52 0.99 0.71 100480 B 2 69.20 173.00 3217.80 1.14 0.82 100480 B 3 112.20 187.00 3440.80 1.24 0.89 100480 B 4 130.72 163.40 2973.88 1.35 0.96 100480 B 5 155.80 155.80 2804.40 1.12 0.80 100480 C 1 31.16 155.80 2929.04 1.25 0.90 100480 C 2 56.00 140.00 2604.00 1.30 0.93 100480 C 3 95.16 158.60 2918.24 1.45 1.03 100480 C 4 157.76 197.20 3589.04 1.56 1.12 100480 C 5 162.60 162.60 2926.80 1.55 1.11 100480 C 6 149.00 1.40 1.00 100480 D 1 29.76 148.80 2797.44 1.44 1.03 100480 D 2 73.68 184.20 3426.12 1.39 0.99 100480 D 3 93.78 156.30 2875.92 1.39 1.00 100480 D 4 131.36 164.20 2988.44 1.34 0.96 100480 D 5 145.10 145.10 2611.80 1.31 0.94

TABLE 14 library Shell- Shell- Shell- ID row column EP-11 EP-12bis EP-8 Shell-EP-9 bead-pi-4 bead-pi-5 ethyl acetate methanol toluene BC(mmol/gr) SI(−) 100484 A 1 3.41 170.70 3239.89 1.89 1.36 100484 A 2 10.50 161.50 3058.00 1.78 1.29 100484 A 3 20.14 183.10 3458.76 1.82 1.31 100484 A 4 21.39 138.00 2600.61 1.36 0.98 100484 A 5 32.66 163.30 3070.04 1.29 0.93 100484 B 1 3.17 158.30 3004.53 1.85 1.33 100484 B 2 10.22 157.20 2976.58 1.93 1.39 100484 B 3 19.91 181.00 3419.09 1.83 1.32 100484 B 4 25.05 161.60 3045.35 1.89 1.36 100484 B 5 29.72 148.60 2793.68 1.91 1.38 100484 C 1 2.99 149.50 2837.51 2.09 1.51 100484 C 2 10.04 154.40 2923.56 1.95 1.41 100484 C 3 19.93 181.20 3422.87 1.83 1.32 100484 C 4 25.76 166.20 3132.04 1.80 1.30 100484 C 5 30.60 153.00 2876.40 1.85 1.33 100484 C 6 211.50 1.39 1.00 100484 D 1 3.07 153.30 2909.63 1.39 1.00 100484 D 2 12.33 189.70 3591.97 1.88 1.35 100484 D 3 18.22 165.60 3128.18 1.76 1.27 100484 D 4 27.27 175.90 3314.84 1.77 1.27 100484 D 5 32.16 160.80 3023.04 1.83 1.32 100485 A 1 3.05 152.36 2318.55 573.15 1.29 0.92 100485 A 2 9.71 149.33 2265.81 561.77 1.03 0.73 100485 A 3 17.53 159.33 2410.31 599.37 1.04 0.74 100485 A 4 25.50 164.51 2481.38 618.89 100485 A 5 35.53 177.66 2671.67 668.34 0.89 0.63 100485 B 1 3.34 167.03 3170.31 1.39 0.98 100485 B 2 8.54 131.44 2488.80 0.69 0.49 100485 B 3 16.87 153.38 2897.42 1.12 0.80 100485 B 4 23.64 152.48 2873.50 1.49 1.06 100485 B 5 33.68 168.42 3166.30 1.55 1.10 100485 C 1 3.06 152.78 2899.67 1.31 0.93 100485 C 2 9.44 145.19 2749.25 1.50 1.06 100485 C 3 16.51 150.09 2835.14 1.49 1.05 100485 C 4 24.86 160.40 3022.70 1.42 1.01 100485 C 5 28.96 144.82 2722.54 1.36 0.96 100485 C 6 139.29 524.01 1.41 1.00 100485 D 1 3.02 150.99 2865.79 1.45 1.03 100485 D 2 10.35 159.29 3016.06 1.48 1.05 100485 D 3 22.61 205.53 3882.41 1.02 0.73 100485 D 4 23.91 154.29 2907.54 1.24 0.88 100485 D 5 28.81 144.06 2708.33 0.82 0.58

TABLE 15 Library: Plate 1 (ID: 100500) Unit: mg Row Col bead-pi-4 toluene Shell-EP-12 BC(mmol/gr) SI(−) 1.00 1.00 171.99 3233.41 34.40 1.31 1.26 1.00 2.00 144.02 2678.73 57.61 0.93 0.90 1.00 3.00 152.57 2807.20 91.54 1.15 1.10 1.00 4.00 156.60 2850.07 125.28 0.71 0.68 1.00 5.00 156.32 2813.83 156.32 0.78 0.75 2.00 1.00 156.72 2946.39 31.34 1.39 1.33 2.00 2.00 156.74 2915.44 62.70 1.61 1.54 2.00 3.00 154.35 2840.04 92.61 2.08 1.99 2.00 4.00 154.35 2809.17 123.48 0.53 0.50 2.00 5.00 153.59 2764.69 153.59 0.60 0.57 2.00 6.00 140.87 529.93 0.00 0.89 0.86 3.00 1.00 140.18 2635.29 28.04 1.15 1.11 3.00 2.00 148.66 2765.06 59.46 1.84 1.77 3.00 3.00 142.44 2620.95 85.47 1.72 1.65 3.00 4.00 149.00 2711.71 119.20 2.27 2.18 3.00 5.00 137.05 2466.83 137.05 0.73 0.70 3.00 6.00 153.03 575.67 0.00 1.04 1.00 4.00 1.00 141.54 2660.95 28.31 1.24 1.19 4.00 2.00 148.47 2761.54 59.39 1.60 1.54 4.00 3.00 130.16 2394.91 78.09 1.17 1.12 4.00 4.00 137.76 2507.23 110.21 1.37 1.31 4.00 5.00 140.22 2523.91 140.22 0.96 0.93

TABLE 16 Library: Plate 2 (ID: 100501) Unit: mg Row Col bead-pi-4 toluene Shell-EP-16 BC(mmol/gr) SI(−) 1.00 1.00 150.72 2833.48 30.14 0.94 0.87 1.00 2.00 150.15 2792.79 60.06 1.22 1.12 1.00 3.00 143.91 2648.00 86.35 1.33 1.23 1.00 4.00 153.43 2792.35 122.74 1.81 1.67 1.00 5.00 154.94 2788.88 154.94 1.01 0.94 2.00 1.00 150.32 2825.98 30.06 1.00 0.93 2.00 2.00 149.12 2773.65 59.65 1.44 1.33 2.00 3.00 149.18 2744.99 89.51 1.93 1.78 2.00 4.00 147.19 2678.84 117.75 1.49 1.38 2.00 5.00 147.82 2660.74 147.82 0.92 0.85 2.00 6.00 147.27 554.03 0.00 0.92 0.85 3.00 1.00 140.45 2640.42 28.09 0.96 0.88 3.00 2.00 141.39 2629.91 56.56 1.50 1.39 3.00 3.00 140.51 2585.40 84.31 0.96 0.89 3.00 4.00 149.00 2711.71 119.20 1.76 1.63 3.00 5.00 131.23 2362.12 131.23 0.00 3.00 6.00 150.21 565.09 0.00 1.08 1.00 4.00 1.00 149.21 2805.05 29.84 0.98 0.91 4.00 2.00 151.98 2826.77 60.79 1.33 1.23 4.00 3.00 155.36 2858.59 93.21 1.40 1.30 4.00 4.00 173.78 3162.71 139.02 2.00 1.85 4.00 5.00 144.52 2601.40 144.52 2.10 1.94

TABLE 17 Library: Plate 2 (ID: 100487) Unit: mg Row Col bead-pi-4 toluene Shell-EP-9 Shell-EP-2 Shell-EP-14 ethyl acetate BC(mmol/gr) SI(−) 1.00 1.00 164.43 3091.28 8.22 24.66 0.00 0.00 0.66 0.87 1.00 2.00 145.70 2709.98 14.57 43.71 0.00 0.00 0.27 0.36 1.00 3.00 147.57 2715.23 22.14 66.41 0.00 0.00 0.26 0.35 1.00 4.00 149.25 2716.30 29.85 89.55 0.00 0.00 0.54 0.71 1.00 5.00 150.19 2703.46 37.55 112.64 0.00 0.00 0.46 0.60 2.00 1.00 143.43 2696.48 9.56 19.12 0.00 0.00 0.57 0.76 2.00 2.00 134.19 2495.93 17.89 35.78 0.00 0.00 0.57 0.76 2.00 3.00 137.07 2522.03 27.41 54.83 0.00 0.00 0.56 0.74 2.00 4.00 134.36 2445.32 35.83 71.66 0.00 0.00 0.53 0.70 2.00 5.00 133.22 2398.03 44.41 88.82 0.00 0.00 0.51 0.68 3.00 1.00 136.77 2386.69 6.84 0.00 20.52 184.64 0.50 0.66 3.00 2.00 151.94 2415.77 15.19 0.00 45.58 410.22 1.14 1.52 3.00 3.00 150.82 2164.30 22.62 0.00 67.87 610.83 1.50 1.98 3.00 4.00 155.74 1993.42 31.15 0.00 93.44 840.97 1.12 1.48 3.00 5.00 157.73 1774.47 39.43 0.00 118.30 1064.68 0.94 1.24 3.00 6.00 153.01 575.59 0.00 0.00 0.00 0.00 0.75 1.00 4.00 1.00 155.30 2733.19 10.35 0.00 20.71 186.35 0.50 0.67 4.00 2.00 150.36 2435.83 20.05 0.00 40.10 360.86 1.03 1.36 4.00 3.00 153.95 2278.47 30.79 0.00 61.58 554.22 1.36 1.80 4.00 4.00 151.60 2031.43 40.43 0.00 80.85 727.68 1.38 1.83 4.00 5.00 151.56 1818.68 50.52 0.00 101.04 909.34 1.24 1.64

TABLE 18 Library: Plate 1 (ID: 100486) Unit: mg Row Col bead-pi-4 toluene Shell-EP-9 Shell-EP-11 Shell-EP-2 Shell-EP-14 ethyl acetate BC(mmol/gr) SI(−) 1.00 1.00 138.29 2599.76 27.66 0.00 0.00 0.00 0.00 0.69 0.95 1.00 2.00 150.40 2797.48 60.16 0.00 0.00 0.00 0.00 0.62 0.85 1.00 3.00 152.42 2804.49 91.45 0.00 0.00 0.00 0.00 0.59 0.82 1.00 4.00 157.56 2867.65 126.05 0.00 0.00 0.00 0.00 0.44 0.61 1.00 5.00 140.22 2523.91 140.22 0.00 0.00 0.00 0.00 0.40 0.56 2.00 1.00 153.01 2876.51 0.00 30.60 0.00 0.00 0.00 0.40 0.55 2.00 2.00 154.37 2871.30 0.00 61.75 0.00 0.00 0.00 0.32 0.44 2.00 3.00 162.50 2989.96 0.00 97.50 0.00 0.00 0.00 0.43 0.60 2.00 4.00 150.26 2734.64 0.00 120.20 0.00 0.00 0.00 0.36 0.49 2.00 5.00 139.04 2502.74 0.00 139.04 0.00 0.00 0.00 0.44 0.61 3.00 1.00 157.29 2957.05 0.00 0.00 31.46 0.00 0.00 0.29 0.40 3.00 2.00 153.91 2862.71 0.00 0.00 61.56 0.00 0.00 0.33 0.45 3.00 3.00 150.84 2775.51 0.00 0.00 90.51 0.00 0.00 0.40 0.55 3.00 4.00 162.02 2948.67 0.00 0.00 129.61 0.00 0.00 0.35 0.49 3.00 5.00 154.41 2779.43 0.00 0.00 154.41 0.00 0.00 0.38 0.53 3.00 6.00 161.45 607.35 0.00 0.00 0.00 0.00 0.00 0.72 1.00 4.00 1.00 156.49 2660.36 0.00 0.00 0.00 31.30 281.69 0.60 0.83 4.00 2.00 157.65 2364.71 0.00 0.00 0.00 63.06 567.53 1.38 1.91 4.00 3.00 157.12 2042.59 0.00 0.00 0.00 94.27 848.46 1.46 2.02 4.00 4.00 153.24 1685.61 0.00 0.00 0.00 122.59 1103.31 1.37 1.89 4.00 5.00 155.00 1395.01 0.00 0.00 0.00 155.00 1395.01 1.20 1.66

Example 5

Binding Capacity Measurements in a non Interfering Buffer

An aliquot of dried resin of weight P(gr), is mixed under gentle agitation with a fixed volume, V(ml), of a phosphate ion solution of concentration C_(start)(mM) buffered at pH 6.5. After resin equilibration, the solution is decanted by centrifugation and the supernatant analyzed for residual phosphate concentration by ionic chromatography, C_(eq)(mM). The binding capacity is calculated as BC (mmol/gr)=V. (C_(start)−C_(eq))/P.

Binding Capacity in a Ex-Vivo Aspirates

In this example healthy patients are given a meal of the same composition as the one prepared for the digestion mimic and aliquots of chyme are then sampled using a tube placed in the lumen of the small intestine.

Normal subjects are intubated with a double lumen polyvinyl tube, with a mercury weighted bag attached to the end of the tube to facilitate movement of the tube into the small intestine. One aspiration aperture of the double lumen tube is located in the stomach and the other aperture is at the Ligament of Treitz (in the upper jejunum). Placement takes place with the use of fluoroscopy.

After correct tube is placed, 550 mL of a liquid standard test meal (supplemented with a marker, polyethylene glycol (PEG)—2 g/550 mL) is infused into the stomach through the gastric aperture at a rate of 22 mL per minute. It requires approximately 25 minutes for the entire meal to reach the stomach. This rate of ingestion simulates the duration of time required to eat normal meals.

Jejunal chyme is aspirated from the tube whose lumen is located at the Ligament of Treitz. This fluid is collected continuously during 30-minute intervals for a two and a half hour period. This results in 5 specimens that are mixed, measured for volume, and lyophilized.

The phosphate binding procedure is identical to the one described earlier with the non-interfering buffer experiment, except that the ex-vivo aspirate liquid is used (after reconstitution of the freeze-dried material in the proper amount of de-ionized water). The binding capacity in the ex-vivo aspirate (VA) is calculated in the same way. Core-shell compositions bind more phosphate than the corresponding core component.

All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

It will be apparent to one of ordinary skill in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the appended claims. 

1. A method of treating an animal subject suffering from a disease selected from the group consisting of renal insufficiency, renal failure, end stage renal disease (ESRD) and combinations thereof, comprising administering to an animal subject in need thereof an effective amount of the pharmaceutical composition of an oral or rectal pharmaceutical composition comprising core-shell particles and a pharmaceutically acceptable excipient, said core-shell particles comprising a core component and a shell component, the core component comprising a potassium-binding cation exchange polymer, the shell component comprising a crosslinked polymer having a thickness ranging from about 0.002 microns to about 50 microns, the shell component being essentially not disintegrated during residence and passage through the gastro-intestinal tract of an animal subject.
 2. A method of treating an animal subject suffering from a disease selected from the group consisting of renal insufficiency, renal failure, end stage renal disease (ESRD) and combinations thereof, comprising administering to an animal subject in need thereof an effective amount of the pharmaceutical composition of an oral or rectal pharmaceutical composition comprising core-shell particles and a pharmaceutically acceptable excipient, said core-shell particles comprising a core component and a shell component, the core component comprising a potassium-binding cation exchange polymer, the shell component comprising a crosslinked polymer, the weight ratio of the shell component polymer to the core component polymer ranging from about 0.0001: 1 to about 0.5:1, the shell component being essentially not disintegrated during residence and passage through the gastro-intestinal tract of an animal subject.
 3. The method of claim 1 wherein said core-shell particle is about 200 nm to about 2 mm in size.
 4. The method of claim 1 wherein said shell component polymer has a thickness ranging from about 0.005 μm to less than about 10 μm.
 5. The method of claim 1 wherein said shell component polymer has a thickness ranging from more than about 1 gm to less than about 10 μm.
 6. The method of claim 1 wherein the core component comprises a cation-exchange polymer comprising acidic functional groups.
 7. The method of claim 5 wherein the core component comprises a cation-exchange polymer comprising functional groups selected from the group consisting of carboxylate, phosphonate, sulfate, sulfonate, sulfamate and combinations thereof
 8. The method of claim 1 wherein the shell component comprises a crosslinked synthetic polymer.
 9. The method of claim 1 wherein the shell component comprises a polymer produced by polymerization of an ethylenic monomer.
 10. The method of claim 1 wherein the shell component comprises a polymer produced by polymerization of a vinylic monomer.
 11. The method of claim 7 wherein the shell component comprises a polymer produced by polymerization of an acrylic or methacrylic monomer.
 12. The method of claim 1 wherein the shell component comprises a hydrophobic polymer, and is essentially not disintegrated during residence and passage of the core-shell particles through the gastro-intestinal tract of a human subject, and wherein the core-shell particles bind potassium ion and retain bound potassium ion during residence and passage through the gastrointestinal tract of the human subject, such that potassium ion is removed from the gastrointestinal tract of the human subject by the core-shell particles to obtain a therapeutic and/or prophylactic benefit.
 13. The method of claim 2 wherein the weight ratio of the shell component polymer to the core component polymer ranges from about 0.002:1 to about 0.1:1.
 14. The method of claim 2 wherein the core component comprises a cation-exchange polymer comprising acidic functional groups.
 15. The method of claim 2 wherein the core component comprises a cation-exchange polymer comprising functional groups selected from the group consisting of carboxylate, phosphonate, sulfate, sulfonate, sulfamate and combinations thereof
 16. The method of claim 2 wherein the shell component comprises a crosslinked synthetic polymer.
 17. The method of claim 2 wherein the shell component comprises a polymer produced by polymerization of an ethylenic monomer.
 18. The method of claim 2 wherein the shell component comprises a polymer produced by polymerization of a vinylic monomer.
 19. The method of claim 2 wherein the shell component comprises a polymer produced by polymerization of an acrylic or methacrylic monomer.
 20. The method of claim 2 wherein the shell component comprises a hydrophobic polymer, and is essentially not disintegrated during residence and passage of the core-shell particles through the gastro-intestinal tract of a human subject, and wherein the core-shell particles bind potassium ion and retain bound potassium ion during residence and passage through the gastrointestinal tract of the human subject, such that potassium ion is removed from the gastrointestinal tract of the human subject by the core-shell particles to obtain a therapeutic and/or prophylactic benefit.
 21. The method of claim 2 wherein the core component comprises a crosslinked cation-exchange polymer comprising acidic functional groups, and the shell component comprises a crosslinked synthetic polymer.
 22. The method of claim 21 wherein the shell component comprises a polymer produced by polymerization of an acrylic or methacrylic monomer. 